Synthetic lethality: General principles, utility and detection using genetic screens in human cells
Open Access
- 19 November 2010
- journal article
- review article
- Published by Wiley in FEBS Letters
- Vol. 585 (1), 1-6
- https://doi.org/10.1016/j.febslet.2010.11.024
Abstract
Synthetic lethality occurs when the simultaneous perturbation of two genes results in cellular or organismal death. Synthetic lethality also occurs between genes and small molecules, and can be used to elucidate the mechanism of action of drugs. This area has recently attracted attention because of the prospect of a new generation of anti-cancer drugs. Based on studies ranging from yeast to human cells, this review provides an overview of the general principles that underlie synthetic lethality and relates them to its utility for identifying gene function, drug action and cancer therapy. It also identifies the latest strategies for the large-scale mapping of synthetic lethalities in human cells which bring us closer to the generation of comprehensive human genetic interaction maps.Funding Information
- Austrian Science Fund (FWF)
- Vienna Science and Technology Fund (WWTF)
This publication has 63 references indexed in Scilit:
- Requirement for NF-κB signalling in a mouse model of lung adenocarcinomaNature, 2009
- Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1Nature, 2009
- Robustness: mechanisms and consequencesTrends in Genetics, 2009
- Synthetic lethal targeting of PTEN mutant cells with PARP inhibitorsEMBO Molecular Medicine, 2009
- A Genome-wide RNAi Screen Identifies Multiple Synthetic Lethal Interactions with the Ras OncogeneCell, 2009
- Principles of Cancer Therapy: Oncogene and Non-oncogene AddictionCell, 2009
- Functional Organization of the S. cerevisiae Phosphorylation NetworkCell, 2009
- A Molecule Targeting VHL-Deficient Renal Cell Carcinoma that Induces AutophagyCancer Cell, 2008
- A synthetic lethal siRNA screen identifying genes mediating sensitivity to a PARP inhibitorThe EMBO Journal, 2008
- A loss-of-function RNA interference screen for molecular targets in cancerNature, 2006