Autoantibodies to lipids in bronchoalveolar lavage fluid of patients with acute respiratory distress syndrome

Abstract

To investigate the presence of autoantibodies to lipids in the bronchoalveolar lavage (BAL) fluid from adult patients with acute respiratory distress syndrome (ARDS). Analysis of immunoglobulin G (IgG) in BAL fluid by electrophoresis followed by immunoblotting and characterization of immunoglobulins as antilipid autoantibodies. Intensive care unit of a university hospital and two research university laboratories. Twenty-seven mechanically ventilated patients in total, including nine patients with ARDS and two control groups. Patients were ventilated with a mechanical ventilation mode. Six aliquots of 20-mL sterile normal saline at 37°C were infused through the working channel of the bronchoscope. Total protein, detection of IgG by electrophoresis followed by immunoblotting, and characterization of IgG by enzyme-linked immunosorbent assay using different lipids as target antigens. Antiphospholipid autoantibodies are present in BAL fluid of ARDS patients. Among the phospholipids tested, phosphatidic acid and phosphatidylserine gave the most significant activity. The IgG fraction, purified from BAL fluids by affinity chromatography, gave the same pattern of binding as that of the BAL fluid. The presence of antiphospholipid autoantibodies in BAL fluid suggests involvement of autoimmune mechanisms in the pathogenesis of ARDS.