The potential of carboxypeptidase G2: antibody conjugates as anti-tumour agents. II. In vivo localising and clearance properties in a choriocarcinoma model

Abstract

The in vivo localising and clearance properties of conjugates of the folate-degrading enzyme caboxypeptidase G2 (CPG2) with anti-human chorionic gonadotrophin (W14A) were measured in nude mice bearing CC3 choriocarcinoma xenografts. Conjugates of W14A-F(ab'')2 fragment coupled to CPG2 localised in tumour as effectively as native antibody alone but showed lower uptake in other major tissues. The clearance rates of conjugates prepared with intact antibody or F(ab'')2 fragment were shown to be up to fire-fold faster than for native antibody and two-fold compared to F(ab'')2 fragment. Molecular weight analysis of residual conjugate in the blood showed that no degradation of conjugate to its component molecules occurred during circulation. It was concluded that F(ab''2): CPG2 conjugates offered the greatest potential for targeting applications.