Magnetic Field Effects on B 12 Ethanolamine Ammonia Lyase: Evidence for a Radical Mechanism
- 18 February 1994
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 263 (5149), 958-960
- https://doi.org/10.1126/science.8310292
Abstract
A change in radical pair recombination rates is one of the few mechanisms by which a magnetic field can interact with a biological system. The kinetic parameter Vmax/Km (where Km is the Michaelis constant) for the coenzyme B12-dependent enzyme ethanolamine ammonia lyase was decreased 25 percent by a static magnetic field near 0.1 tesla (1000 gauss) with unlabeled ethanolamine and decreased 60 percent near 0.15 tesla with perdeuterated ethanolamine. This effect is likely caused by a magnetic field-induced change in intersystem crossing rates between the singlet and triplet spin states in the [cob(II)alamin:5'-deoxyadenosyl radical] spin-correlated radical pair.Keywords
This publication has 29 references indexed in Scilit:
- Magnetic and spin effects in photoreduction of uranyl saltsAccounts of Chemical Research, 1991
- PROTEIN RADICAL INVOLVEMENT IN BIOLOGICAL CATALYSIS?Annual Review of Biochemistry, 1989
- Magnetic field effects in chemical kinetics and related phenomenaChemical Reviews, 1989
- The B12-dependent isomerase enzymes; how the protein controls the active siteChemical Society Reviews, 1985
- Cobalt-carbon bond dissociation energy of coenzyme B12Journal of the American Chemical Society, 1984
- Thermolysis of adenosylcobalamin: a product, kinetic, and cobalt-carbon (C5') bond dissociation energy studyInorganic Chemistry, 1984
- Use of large magnetic fields to probe photoinduced electron-transfer reactions: an example from photosynthetic reaction centersJournal of the American Chemical Society, 1982
- Use of Isotope Effects to Elucidate Enzyme MechanismCritical Reviews in Biochemistry, 1982
- A Spectrophotometric Rapid Kinetic Study of Reactions Catalysed by Coenzyme‐B12‐Dependent Ethanolamine Ammonia‐LyaseEuropean Journal of Biochemistry, 1978
- On the magnetic field dependence of the yield of the triplet state in reaction centers of photosynthetic bacteriaBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977