The STT3a Subunit Isoform of the Arabidopsis Oligosaccharyltransferase Controls Adaptive Responses to Salt/Osmotic Stress
- 1 October 2003
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 15 (10), 2273-2284
- https://doi.org/10.1105/tpc.013862
Abstract
Arabidopsis stt3a-1 and stt3a-2 mutations cause NaCl/osmotic sensitivity that is characterized by reduced cell division in the root meristem. Sequence comparison of the STT3a gene identified a yeast ortholog, STT3, which encodes an essential subunit of the oligosaccharyltransferase complex that is involved in protein N-glycosylation. NaCl induces the unfolded protein response in the endoplasmic reticulum (ER) and cell cycle arrest in root tip cells of stt3a seedlings, as determined by expression profiling of ER stress–responsive chaperone (BiP-GUS) and cell division (CycB1;1-GUS) genes, respectively. Together, these results indicate that plant salt stress adaptation involves ER stress signal regulation of cell cycle progression. Interestingly, a mutation (stt3b-1) in another Arabidopsis STT3 isogene (STT3b) does not cause NaCl sensitivity. However, the stt3a-1 stt3b-1 double mutation is gametophytic lethal. Apparently, STT3a and STT3b have overlapping and essential functions in plant growth and developmental processes, but the pivotal and specific protein glycosylation that is a necessary for recovery from the unfolded protein response and for cell cycle progression during salt/osmotic stress recovery is associated uniquely with the function of the STT3a isoform.Peer revieweKeywords
This publication has 55 references indexed in Scilit:
- The cellulose‐deficient Arabidopsis mutant rsw3 is defective in a gene encoding a putative glucosidase II, an enzyme processing N‐glycans during ER quality controlThe Plant Journal, 2002
- An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiaeJournal of Biological Chemistry, 2002
- C-terminal domain phosphatase-like family members (AtCPLs) differentially regulateArabidopsis thalianaabiotic stress signaling, growth, and developmentProceedings of the National Academy of Sciences of the United States of America, 2002
- Translocation of lipid-linked oligosaccharides across the ER membrane requires Rft1 proteinNature, 2002
- Regulation of Cell Cycle Progression by Swe1p and Hog1p Following Hypertonic StressMolecular Biology of the Cell, 2001
- Floral Spray Transformation Can Efficiently Generate ArabidopsisTransgenic Research, 2000
- Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controlsGenes & Development, 1999
- An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutantThe Plant Journal, 1997
- The STT3 protein is a component of the yeast oligosaccharyltransferase complexMolecular Genetics and Genomics, 1997
- Interaction of the Thiol-Dependent Reductase ERp57 with Nascent GlycoproteinsScience, 1997