Use of Heat Stress Responsive Gene Expression Levels for Early Selection of Heat Tolerant Cabbage (Brassica oleracea L.)
Open Access
- 3 June 2013
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 14 (6), 11871-11894
- https://doi.org/10.3390/ijms140611871
Abstract
Cabbage is a relatively robust vegetable at low temperatures. However, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. Thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. Easier or faster selection of superior varieties of cabbage, which are tolerant to heat and disease and have improved taste and quality, can be achieved with molecular and biological methods. We compared heat-responsive gene expression between a heat-tolerant cabbage line (HTCL), “HO”, and a heat-sensitive cabbage line (HSCL), “JK”, by Genechip assay. Expression levels of specific heat stress-related genes were increased in response to high-temperature stress, according to Genechip assays. We performed quantitative RT-PCR (qRT-PCR) to compare expression levels of these heat stress-related genes in four HTCLs and four HSCLs. Transcript levels for heat shock protein BoHsp70 and transcription factor BoGRAS (SCL13) were more strongly expressed only in all HTCLs compared to all HSCLs, showing much lower level expressions at the young plant stage under heat stress (HS). Thus, we suggest that expression levels of these genes may be early selection markers for HTCLs in cabbage breeding. In addition, several genes that are involved in the secondary metabolite pathway were differentially regulated in HTCL and HSCL exposed to heat stress.Keywords
This publication has 60 references indexed in Scilit:
- Chloroplastic Hsp100 chaperones ClpC2 and ClpD interact in vitro with a transit peptide only when it is located at the N-terminus of a proteinBMC Plant Biology, 2012
- Regulation of survival gene hsp70Cell Stress and Chaperones, 2012
- Small heat shock proteins and α-crystallins: dynamic proteins with flexible functionsTrends in Biochemical Sciences, 2011
- agriGO: a GO analysis toolkit for the agricultural communityNucleic Acids Research, 2010
- Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthersJournal of Experimental Botany, 2009
- A Heat-Inducible Transcription Factor, HsfA2, Is Required for Extension of Acquired Thermotolerance in ArabidopsisPlant Physiology, 2006
- Could Heat Shock Transcription Factors Function as Hydrogen Peroxide Sensors in Plants?Annals of Botany, 2006
- Molecular Chaperones and Protein Quality ControlCell, 2006
- Role of plant heat-shock proteins and molecular chaperones in the abiotic stress responseTrends in Plant Science, 2004
- Hsp90 as a capacitor of phenotypic variationNature, 2002