Multistate Evaluation of an Ultrafiltration-Based Procedure for Simultaneous Recovery of Enteric Microbes in 100-Liter Tap Water Samples
Open Access
- 1 July 2007
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (13), 4218-4225
- https://doi.org/10.1128/aem.02713-06
Abstract
Ultrafiltration (UF) is increasingly being recognized as a potentially effective procedure for concentrating and recovering microbes from large volumes of water and treated wastewater. Because of their very small pore sizes, UF membranes are capable of simultaneously concentrating viruses, bacteria, and parasites based on size exclusion. In this study, a UF-based water sampling procedure was used to simultaneously recover representatives of these three microbial classes seeded into 100-liter samples of tap water collected from eight cities covering six hydrologic areas of the United States. The UF-based procedure included hollow-fiber UF as the primary step for concentrating microbes and then used membrane filtration for bacterial culture assays, immunomagnetic separation for parasite recovery and quantification, and centrifugal UF for secondary concentration of viruses. Water samples were tested for nine water quality parameters to investigate whether water quality data correlated with measured recovery efficiencies and molecular detection levels. Average total method recovery efficiencies were 71, 97, 120, 110, and 91% for φX174 bacteriophage, MS2 bacteriophage, Enterococcus faecalis , Clostridium perfringens spores, and Cryptosporidium parvum oocysts, respectively. Real-time PCR and reverse transcription-PCR (RT-PCR) for seeded microbes and controls indicated that tap water quality could affect the analytical performance of molecular amplification assays, although no specific water quality parameter was found to correlate with reduced PCR or RT-PCR performance.Keywords
This publication has 20 references indexed in Scilit:
- Evaluation of 1MDS electropositive microfilters for simultaneous recovery of multiple microbe classes from tap waterJournal of Microbiological Methods, 2007
- Development and Evaluation of a Broadly Reactive TaqMan Assay for Rapid Detection of Hepatitis A VirusApplied and Environmental Microbiology, 2005
- Comparison of 2 ultrafiltration systems for the concentration of seeded viruses from environmental watersCanadian Journal of Microbiology, 2005
- Real-Time PCR for Quantification of Giardia and Cryptosporidium in Environmental Water Samples and SewageApplied and Environmental Microbiology, 2003
- Effects of pH and Magnetic Material on Immunomagnetic Separation of Cryptosporidium Oocysts from Concentrated Water SamplesApplied and Environmental Microbiology, 2002
- Non-perturbative gauge groups and local mirror symmetryJournal of High Energy Physics, 2001
- Improved Purification Methods for Calf‐Derived Cryptosporidium parvum Oocysts Using Discontinuous Sucrose and Cesium Chloride GradientsThe Journal of Eukaryotic Microbiology, 1996
- The removal of phages T1 and PP7, and poliovirus from fluids with hollow-fiber ultrafilters with molecular weight cut-offs of 50 000, 13 000, and 6000Canadian Journal of Microbiology, 1995
- Replication Kinetics and Cytopathic Effect of Hepatitis A VirusJournal of General Virology, 1989
- Virus concentration using hollow fiber membranesWater Research, 1975