ISOLATION AND MOLECULAR CHARACTERIZATION OF BRAIN MICROVASCULAR ENDOTHELIAL CELLS FROM HUMAN BRAIN TUMORS
- 1 January 2002
- journal article
- Published by Springer Science and Business Media LLC in In Vitro Cellular & Developmental Biology – Animal
- Vol. 38 (5), 273-281
- https://doi.org/10.1290/1071-2690(2002)038<0273:iamcob>2.0.co;2
Abstract
Brain tumor formation and growth is accompanied by the proliferation and infiltration of blood capillaries. The phenotypes of endothelial cells that make up capillaries are known to differ not only in the tissues in which endothelial cells are located but also as a result of the microenvironment to which they are exposed. For this reason, primary cultures of brain endothelial cells were isolated from human brain tumors removed by surgery and compared with cells from normal tissue. The primary confluent monolayers that grew out of isolated capillary fragments consisted of closely associated, elongated, fusiform-shaped cells. But brain tumor-derived endothelial cells in culture exhibited significantly less expression of endothelial-specific Factor VIII-related antigen compared with cells isolated from normal tissue. Cultured cells that exhibited binding of Ulex europaeus lectin were shown to take up Dil-Ac-Ldl and formed continuous monolayers that were joined together by tight junctions. The cells also exhibited characteristics of the cells of the brain microvasculature in vitro as seen by the presence of large numbers of mitochondria and few pinocytotic vesicles and by the absence of Weibel-Palade bodies within the cells. The expression of vascular cell adhesion molecule-1, E-Selectin, and the tight junction associated protein ZO-1 but not intercellular adhesion molecule-1 was demonstrated by immunohistological staining or reverse transcriptase-polymerase chain reaction methodologies. Comparative studies of these endothelial cells with endothelial cells from normal tissue will be useful for determining and understanding how the blood-brain barrier differs and functions in tumor and healthy tissues and may lead to strategies for brain tumor therapeutic approaches.Keywords
This publication has 45 references indexed in Scilit:
- Hydrocortisone Reinforces the Blood–Brain Barrier Properties in a Serum Free Cell Culture SystemBiochemical and Biophysical Research Communications, 1998
- Molecular changes during the genesis of human gliomasSeminars in Surgical Oncology, 1998
- Feline immunodeficiency virus can productively infect cultured endothelial cells from cat brain microvesselsJournal of General Virology, 1994
- Upregulation of the low density lipoprotein receptor at the blood-brain barrier: intercommunications between brain capillary endothelial cells and astrocytes.The Journal of cell biology, 1994
- Protamine-mediated transport of albumin into brain and other organs of the rat. Binding and endocytosis of protamine-albumin complex by microvascular endothelium.JCI Insight, 1993
- The Cerebral Microvessels in Culture, an UpdateJournal of Neurochemistry, 1992
- Induction of γ-glutamyl transpeptidase in cultured cerebral endothelial cells by a product released by astrocytesBrain Research, 1987
- Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein.The Journal of cell biology, 1984
- Brain microvessel endothelial cells in tissue culture: A model for study of blood‐brain barrier permeabilityAnnals of Neurology, 1983
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJCI Insight, 1973