Complexation between Copper(II) and Glycine in Aqueous Acid Solutions by Window Factor Analysis of Visible Spectra

Abstract

Window factor analysis (WFA), a self-modeling chemometric technique, is used to extract the concentration profiles of the complexes formed between Cu(II) and glycine (GlyH) during pH titration in the acid region. The visible absorption spectra of aqueous acid solutions, each containing 0.0020 M Cu(II) and 0.500 M glycine, are recorded at various pHs, ranging from 1 to 7. The concentration profiles and the spectral absorptivities of four copper species, Cu²⁺, CuGlyH²⁺, CuGly⁺, and CuGly₂, are obtained. The spectral absorptivities of the complexes are in good agreement with those reported in the literature. The concentration profiles are used to determine the stepwise equilibrium constants of the three complexes. The reason why rank annihilation evolving factor analysis (RAEFA), a precursor technique, was not successful in resolving the spectral data is explained.