Tobacco mosiac virus particle structure and the initiation of disassembly
Open Access
- 29 March 1999
- journal article
- review article
- Published by The Royal Society in Philosophical Transactions Of The Royal Society B-Biological Sciences
- Vol. 354 (1383), 551-557
- https://doi.org/10.1098/rstb.1999.0406
Abstract
The structure of an intact tobacco mosaic virus (TMV) particle was determined at 2.9 Å resolution using fibre diffraction methods. All residues of the coat protein and the three nucleotides of RNA that are bound to each protein subunit were visible in the electron density map. Examination of the structures of TMV, cucumber green mottle mosaic virus and ribgrass mosaic virus, and site–directed mutagenesis experiments in which carboxylate groups were changed to the corresponding amides, showed that initial stages of disassembly are driven by complex electrostatic interactions involving at least seven carboxylate side–chains and a phosphate group. The locations of these interactions can drift during evolution, allowing the viruses to evade plant defensive responses that depend on recognition of the viral coat protein surface.Keywords
This publication has 30 references indexed in Scilit:
- Structure of ribgrass mosaic virus at 2.9 å resolution evolution and taxonomy of tobamovirusesJournal of Molecular Biology, 1997
- Structure Determination of Cucumber Green Mottle Mosaic Virus by X-ray Fiber Diffraction: Significance for the Evolution of TobamovirusesJournal of Molecular Biology, 1994
- Structure of the U2 strain of tobacco mosaic virus refined at 3.5 Å resolution using X-ray fiber diffractionJournal of Molecular Biology, 1992
- Visualization of protein-nucleic acid interactions in a virusJournal of Molecular Biology, 1989
- Calcium ion binding by tobacco mosaic virusJournal of Molecular Biology, 1983
- Structure of the RNA in tobacco mosaic virusJournal of Molecular Biology, 1981
- Structures and roles of the polymorphic forms of tobacco mosaic virus: V. Conservation of the abnormally titrating groups in tobacco mosaic virusJournal of Molecular Biology, 1972
- Structures and roles of the polymorphic forms of tobacco mosaic virus protein: IV. Control of mode of aggregation of tobacco mosaic virus protein by proton bindingJournal of Molecular Biology, 1972
- The structure of tobacco mosaic virusBiochimica et Biophysica Acta, 1954
- Infra-Red Dichroism and Protein Structure: Infra-Red Dichroism of Tobacco Mosaic Virus NucleoproteinNature, 1952