Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules
- 28 March 2019
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 116 (16), 7899-7904
- https://doi.org/10.1073/pnas.1818553116
Abstract
We present a simple nanopore-electroporation (NanoEP) platform for delivery of nucleic acids, functional protein, and Cas9 single-guide RNA ribonucleoproteins into both adherent and suspension cells with up to 80% delivery efficiency and >95% cell viability. Low-voltage electric pulses permeabilize a small area of cell membrane as a cell comes into close contact with the nanopores. The biomolecule cargo is then electrophoretically drawn into the cells through the nanopores. In addition to high-performance delivery with low cell toxicity, the NanoEP system does not require specialized buffers, expensive materials, complicated fabrication processes, or cell manipulation; it simply consists of a generic nanopore-embedded water-filter membrane and a low-voltage square-wave generator. Ultimately, the NanoEP platform offers an effective and flexible method for universal intracellular delivery.Keywords
Funding Information
- W. M. Keck Foundation (89208-31150-44-X-IQJED)
- W. M. Keck Foundation (89208-31150-44-X-IQJED)
- W. M. Keck Foundation (89208-31150-44-X-IQJED)
- W. M. Keck Foundation (89208-31150-44-X-IQJED)
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