DIFFERENTIAL EFFECTS OF EXOGENOUS INTERLEUKIN-10 ON CARDIAC ALLOGRAFT SURVIVAL1,2
- 15 November 1999
- journal article
- immunobiology
- Published by Ovid Technologies (Wolters Kluwer Health) in Transplantation
- Vol. 68 (9), 1402-1409
- https://doi.org/10.1097/00007890-199911150-00029
Abstract
Background. There have been conflicting reports of the influence of exogenous mammalian interleukin (IL)-10 on immune reactivity. These findings may reflect the pleiotropic effects of IL-10 on the functions of antigen-presenting cells and immune effector cells. The purpose of this study was to extend observations of the influence of the cytokine on organ allograft survival and to investigate its effects on the function of accessory and immune effector cells in a mouse cardiac transplant model. Methods. C3H (H2k) recipients of heterotopic vascularized B10 (H-2b) heart allografts were treated with recombinant (r) mouse IL-10 over a wide range of doses (0.2-200 μg/day), either before the transplant (days −3, −2, −1), peri-operatively (days −1, 0, 1), or after the transplant (days 0-6). Anti-donor cytotoxic T lymphocyte activity of host spleen and graft-infiltrating cells, and circulating complement-dependent cytotoxic antibody titers were determined by isotope release assays. Mixed leukocyte reactions were used to determine the influence of IL-10 on the function of antigen-presenting cells and allogeneic responder T cells. Results. Recipient pre-transplant administration of IL-10 (days −3, −2, −1) prolonged graft survival at all doses tested. Donor pretreatment with IL-10 (25 μg/day; days −3, −2, −1) was also effective, but less. A pre-transplant or perioperative course of IL-10, however, did not significantly affect the immunosuppressive action of tacrolimus given on days 0-6. If given only after the transplant, IL-10 either had no effect on graft survival or (at high dosage) accelerated rejection and prevented the immunosuppressive effect of cyclosporine. Pretransplant treatment of graft recipients with IL-10 reduced splenic anti-donor cytotoxic T lymphocyte activity and the incidence of graft-infiltrating CD8+ cells. There was no significant effect on circulating alloantibody titers. MLR assays revealed that pre-incubation of responder cells, but not stimulator spleen cells with IL-10, inhibited T cell proliferation, whereas addition of IL-10 after the start of culture modestly enhanced proliferation. Preincubation of purified T responders with IL-10 showed no inhibitory effect. Conclusion. The modest and opposing effects of exogenous IL-10 on organ allograft survival are dependent on timing and dosage. Recipient pretreatment prolongs graft survival. This finding, together with the MLR results, suggest that IL-10 inhibits the function of host immune accessory cells and that the direct pathway of alloantigen presentation may be less susceptible to inhibition by IL-10.Keywords
This publication has 23 references indexed in Scilit:
- COMBINATION THERAPY WITH CYCLOSPORINE AND INTERLEUKIN-4 OR INTERLEUKIN-10 PROLONGS SURVIVAL OF SYNGENEIC PANCREATIC ISLET GRAFTS IN NONOBESE DIABETIC MICETransplantation, 1997
- SYSTEMIC ADMINISTRATION OF CELLULAR INTERLEUKIN-10 CAN EXACERBATE CARDIAC ALLOGRAFT REJECTION IN MICE1,2Transplantation, 1996
- Intragraft expression of IL-10 messenger RNA: A novel correlate of renal allograft rejectionKidney International, 1995
- HIGH FREQUENCY OF IL-10-SECRETING CD4+ GRAFT-INFILTRATING T LYMPHOCYTES IN PROMPTLY REJECTED KIDNEY ALLOGRAFTSTransplantation, 1995
- Interleukin 10 pretreatment protects target cells from tumor- and allo-specific cytotoxic T cells and downregulates HLA class I expression.The Journal of Experimental Medicine, 1994
- Human and viral interleukin-10 (hIL-10 and vIL-10) mediate opposing effects in tumor immunityCytokine, 1994
- Interleukin‐10 inhibits B7 and intercellular adhesion molecule‐1 expression on human monocytesEuropean Journal of Immunology, 1994
- Interleukin-10 prevents spontaneous death of germinal center B cells by induction of the bcl-2 protein.JCI Insight, 1994
- Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells.The Journal of Experimental Medicine, 1993
- Interleukin 10 (IL-10) and viral IL-10 strongly reduce antigen-specific human T cell proliferation by diminishing the antigen-presenting capacity of monocytes via downregulation of class II major histocompatibility complex expression.The Journal of Experimental Medicine, 1991