The optogenetic (r)evolution
Open Access
- 20 December 2011
- journal article
- review article
- Published by Springer Science and Business Media LLC in Molecular Genetics and Genomics
- Vol. 287 (2), 95-109
- https://doi.org/10.1007/s00438-011-0663-7
Abstract
Optogenetics is a rapidly evolving field of technology that allows optical control of genetically targeted biological systems at high temporal and spatial resolution. By heterologous expression of light-sensitive microbial membrane proteins, opsins, cell type-specific depolarization or silencing can be optically induced on a millisecond time scale. What started in a petri dish is applicable today to more complex systems, ranging from the dissection of brain circuitries in vitro to behavioral analyses in freely moving animals. Persistent technical improvement has focused on the identification of new opsins, suitable for optogenetic purposes and genetic engineering of existing ones. Optical stimulation can be combined with various readouts defined by the desired resolution of the experimental setup. Although recent developments in optogenetics have largely focused on neuroscience it has lately been extended to other targets, including stem cell research and regenerative medicine. Further development of optogenetic approaches will not only highly increase our insight into health and disease states but might also pave the way for a future use in therapeutic applications.Keywords
This publication has 102 references indexed in Scilit:
- Functional Control of Transplantable Human ESC-Derived Neurons Via Optogenetic TargetingThe International Journal of Cell Cloning, 2010
- fMRI under the spotlightNature, 2010
- Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate BrainNeuron, 2009
- Characterization of Engineered Channelrhodopsin Variants with Improved Properties and KineticsBiophysical Journal, 2009
- Escape Behavior Elicited by Single, Channelrhodopsin-2-Evoked Spikes in Zebrafish Somatosensory NeuronsCurrent Biology, 2008
- eNpHR: a Natronomonas halorhodopsin enhanced for optogenetic applicationsBrain Cell Biology, 2008
- Improved expression of halorhodopsin for light-induced silencing of neuronal activityBrain Cell Biology, 2008
- In Vivo Light-Induced Activation of Neural Circuitry in Transgenic Mice Expressing Channelrhodopsin-2Neuron, 2007
- Ectopic Expression of a Microbial-Type Rhodopsin Restores Visual Responses in Mice with Photoreceptor DegenerationNeuron, 2006
- Light Activation of Channelrhodopsin-2 in Excitable Cells of Caenorhabditis elegans Triggers Rapid Behavioral ResponsesCurrent Biology, 2005