The in vitro study of apoptosis in NB4 cell induced by citral
- 10 May 2012
- journal article
- Published by Springer Science and Business Media LLC in Cytotechnology
- Vol. 65 (1), 49-57
- https://doi.org/10.1007/s10616-012-9453-2
Abstract
Citral, 3,7-dimethyl-2,6-octadienal, is a key component of the essential oils extracted from several lemon-scented herbal plants. Besides its antifungal activity, the anticancer effect of citral was studied in recent years. In this study, we investigated the effect of citral on the acute promyelocytic leukemia cell line NB4. Citral treatment had an antiproliferative effect in NB4 cells via the induction of apoptosis assessed by morphology, proliferation assay, DNA electrophoresis, Annexin V-FITC/PI staining and caspase-3 activation. And citral induced apoptosis of NB4 cells in a dose- and time-dependent manner. In addition, citral treatment induced decreased mitochondrial membrane potential, indicating that citral induced apoptosis via the mitochondrial pathway. Bax up-regulation and Bcl-2 down-regulation on mRNA level and NF-κB down-regulation on protein level was found in this study, suggesting that Bcl-2, Bax and NF-κB may be involved in the mechanism of the apoptotic effect of citral on NB4 cells. These data suggest that citral has a potential therapeutic effect on leukemia.Keywords
This publication has 18 references indexed in Scilit:
- Recent advances in apoptosis, mitochondria and drug resistance in cancer cellsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 2011
- Blocking NF-κB nuclear translocation leads to p53-related autophagy activation and cell apoptosisWorld Journal of Gastroenterology, 2011
- Citral inhibits cell proliferation and induces apoptosis and cell cycle arrest in MCF‐7 cellsFundamental & Clinical Pharmacology, 2009
- Regulation and Function of NF-κB Transcription Factors in the Immune SystemAnnual Review of Immunology, 2009
- NF-κB: linking inflammation and immunity to cancer development and progressionNature Reviews Immunology, 2005
- Citral is a New Inducer of Caspase-3 in Tumor Cell LinesPlanta Medica, 2005
- NF-κB Signals Induce the Expression of c-FLIPMolecular and Cellular Biology, 2001
- Aberrant rel/nfkb genes and activity in human cancerOncogene, 1999
- NF-κB Antiapoptosis: Induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to Suppress Caspase-8 ActivationScience, 1998
- Constitutive nuclear factor-kappaB-RelA activation is required for proliferation and survival of Hodgkin's disease tumor cells.JCI Insight, 1997