Prospective Identification of Enteroviruses Involved in Meningitis in 2006 through Direct Genotyping in Cerebrospinal Fluid
- 1 January 2008
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 46 (1), 87-96
- https://doi.org/10.1128/jcm.01020-07
Abstract
Enterovirus infections were investigated with special emphasis on performing rapid molecular identification of enterovirus serotypes responsible for aseptic meningitis directly in cerebrospinal fluid (CSF). Enterovirus genotyping was carried out directly with specimens tested for the diagnostic procedure, using two seminested PCR assays designed to amplify the complete and partial gene sequences encoding the VP1 and VP4/VP2 capsid proteins, respectively. The method was used for identifying the enterovirus serotypes involved in meningitis in 45 patients admitted in 2005. Enterovirus genotyping was achieved in 98% of the patients studied, and we obtained evidence of 10 of the most frequent serotypes identified earlier by genotyping of virus isolates. The method was applied for the prospective investigation of 54 patients with meningitis admitted consecutively in 2006. The enterovirus serotypes involved were identified with the cerebrospinal fluid (CSF) of 52 patients (96%) and comprised 13 serotypes within the human enterovirus B species and 1 within the human enterovirus A species. The three most common serotypes were echovirus 13 (E13; 24%), E6 (23%), and coxsackievirus B5 (11.5%), a pattern different from that observed in 2005. Genotyping of virus isolates was also performed in 35 patients in 2006 (meningitis, n = 31; other diseases, n = 4). By comparison, direct genotyping in CSF yielded a more complete pattern of enterovirus serotypes, thereby allowing the detection of rare serotypes: three less common serotypes (CB2, E21, and E27) were not detected by indirect genotyping alone. The study shows the feasibility of prospective enterovirus genotyping within 1 week in a laboratory setting.Keywords
This publication has 56 references indexed in Scilit:
- Neurodevelopment and Cognition in Children after Enterovirus 71 InfectionThe New England Journal of Medicine, 2007
- Molecular characterization of enteroviruses isolated from patients with aseptic meningitis in Korea, 2005Archiv für die gesamte Virusforschung, 2007
- High Prevalence of Human Enterovirus A Infections in Natural Circulation of Human EnterovirusesJournal of Clinical Microbiology, 2006
- Sensitive, Seminested PCR Amplification of VP1 Sequences for Direct Identification of All Enterovirus Serotypes from Original Clinical SpecimensJournal of Clinical Microbiology, 2006
- Du diagnostic moléculaire initial prospectif des méningites à entérovirus⋯ à la lutte contre l'antibiorésistanceMedecine Et Maladies Infectieuses, 2006
- Evidence for Frequent Recombination within SpeciesHuman Enterovirus BBased on Complete Genomic Sequences of All Thirty-Seven SerotypesJournal of Virology, 2004
- Molecular Evidence of Persistent Echovirus 13 Meningoencephalitis in a Patient with Relapsed Lymphoma after an Outbreak of Meningitis in 2000Journal of Clinical Microbiology, 2003
- Molecular Identification and Typing of Enteroviruses Isolated from Clinical SpecimensJournal of Clinical Microbiology, 2002
- Genotypic variation in Coxsackievirus B5 isolates from three different outbreaks in the United StatesVirus Research, 1995
- Basic local alignment search toolJournal of Molecular Biology, 1990