MicroRNA, hsa-miR-200c, is an independent prognostic factor in pancreatic cancer and its upregulation inhibits pancreatic cancer invasion but increases cell proliferation
Open Access
- 28 June 2010
- journal article
- Published by Springer Science and Business Media LLC in Molecular Cancer
- Vol. 9 (1), 169
- https://doi.org/10.1186/1476-4598-9-169
Abstract
Recently, the microRNA-200 family was reported to affect cancer biology by regulating epithelial to mesenchymal transition (EMT). Especially, the expression of miR-200c has been shown to be associated with upregulating the expression of E-cadherin, a gene known to be involved in pancreatic cancer behavior. However, the significance of miR-200c in pancreatic cancer is unknown. In the present study, we investigated the relationship between E-cadherin and miR-200c expression in a panel of 14 pancreatic cancer cell lines and in macro-dissected formalin-fixed paraffin-embedded (FFPE) tissue samples obtained from 99 patients who underwent pancreatectomy for pancreatic cancer. We also investigated the effects of miR-200c on the proliferation and invasion of pancreatic cancer cells. We found that patients with high levels of miR-200c expression had significantly better survival rates than those with low levels of miR-200c expression. We also found a remarkably strong correlation between the levels of miR-200c and E-cadherin expression. These data indicate that miR-200c may play a role in the pancreatic cancer biology and may be a novel marker for the prognosis of pancreatic cancer.Keywords
This publication has 41 references indexed in Scilit:
- Loss of miR-200c: A Marker of Aggressiveness and Chemoresistance in Female Reproductive CancersJournal of Oncology, 2009
- Up-regulation of miR-200 and let-7 by Natural Agents Leads to the Reversal of Epithelial-to-Mesenchymal Transition in Gemcitabine-Resistant Pancreatic Cancer CellsCancer Research, 2009
- Downregulation of miRNA-200c Links Breast Cancer Stem Cells with Normal Stem CellsCell, 2009
- Circulating microRNAs as stable blood-based markers for cancer detectionProceedings of the National Academy of Sciences of the United States of America, 2008
- The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2Genes & Development, 2008
- The E-cadherin repressor Snail is associated with lower overall survival of ovarian cancer patientsBritish Journal of Cancer, 2007
- Chromosomal Rearrangements and MicroRNAs: A New Cancer Link with Clinical ImplicationsJCI Insight, 2007
- Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cellsBMC Biotechnology, 2007
- E-cadherin engagement stimulates proliferation via Rac1The Journal of cell biology, 2006
- A microRNA polycistron as a potential human oncogeneNature, 2005