Panel of autoantibodies against multiple tumor‐associated antigens for detecting gastric cancer
Open Access
- 1 March 2017
- journal article
- Published by Wiley in Cancer Science
- Vol. 108 (3), 308-315
- https://doi.org/10.1111/cas.13158
Abstract
Gastric cancer is the second leading cause of cancer death in the world, and effective diagnosis is extremely important for good outcome. We assessed the diagnostic potential of an autoantibody panel that may provide a novel tool for the early detection of gastric cancer. We analyzed data from patients with gastric cancer and normal controls in test and validation cohorts. Autoantibody levels were measured against a panel of six tumor-associated antigens (TAAs) by ELISA: p53, heat shock protein 70, HCC-22-5, peroxiredoxin VI, KM-HN-1, and p90 TAA. We assessed serum autoantibodies in 100 participants in the test cohort. The validation cohort comprised 248 participants. Autoantibodies to at least one of the six antigens showed a sensitivity/specificity of 49.0% (95% confidence interval [CI], 39.2–58.8%)/92.4% (95% CI, 87.2–97.6%), and 52.0% (95% CI, 42.2–61.8%)/90.5% (95% CI, 84.8–96.3%) in the test and validation cohorts, respectively. In the validation cohort, no significant differences were seen when patients were subdivided based on age, sex, depth of tumor invasion, lymph node metastasis, distant metastasis, peritoneal dissemination, or TNM stage. Patients who were positive for more than two antibodies in the panel tended to have a worse prognosis than those who were positive for one or no antibody. Measurement of autoantibody response to multiple TAAs in an optimized panel assay to discriminate patients with early stage gastric cancer from normal controls may aid in the early detection of gastric cancer.Keywords
Funding Information
- Japan Society for the Promotion of Science (15K10117, 16K1052)
This publication has 52 references indexed in Scilit:
- Over‐expression of HSP70 attenuates caspase‐dependent and caspase‐independent pathways and inhibits neuronal apoptosisJournal of Neurochemistry, 2012
- Expression of CIP2A in renal cell carcinomas correlates with tumour invasion, metastasis and patients’ survivalBritish Journal of Cancer, 2011
- Serum-autoantibodies for discovery of prostate cancer specific biomarkersThe Prostate, 2011
- 7th Edition of the AJCC Cancer Staging Manual: StomachAnnals of Surgical Oncology, 2010
- Clinical validation of an autoantibody test for lung cancerAnnals Of Oncology, 2010
- Detection of anti-CUEC-23 antibodies in serum of patients with esophageal squamous cell carcinoma: a possible new serum marker for esophageal cancerThe Esophagus, 2009
- Bortezomib Overcomes Tumor Necrosis Factor-related Apoptosis-inducing Ligand Resistance in Hepatocellular Carcinoma Cells in Part through the Inhibition of the Phosphatidylinositol 3-Kinase/Akt PathwayPublished by Elsevier BV ,2009
- Titration of serum p53 antibodies in patients with gastric cancer: a single-institute study of 40 patientsGastric Cancer, 2005
- Antioxidant enzymes and redox regulating thiol proteins in malignancies of human lungFEBS Letters, 2004