Requirement of the Activation-Induced Deaminase ( AID ) Gene for Immunoglobulin Gene Conversion
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- 15 February 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 295 (5558), 1301-1306
- https://doi.org/10.1126/science.1067308
Abstract
Three phenotypically distinct processes—somatic hypermutation, gene conversion, and switch recombination—remodel the functionally rearranged immunoglobulin (Ig) loci in B cells. Somatic hypermutation and switch recombination have recently been shown to depend on the activation-induced deaminase ( AID ) gene product. Here, we show that the disruption of the AID gene in the chicken B cell line DT40 completely blocks Ig gene conversion and that this block can be complemented by reintroduction of the AID complementary DNA. This demonstrates that the AID master gene controls all B cell–specific modifications of vertebrate Ig genes.Keywords
This publication has 13 references indexed in Scilit:
- The DT40 web site: sampling and connecting the genes of a B cell lineNucleic Acids Research, 2002
- Activation-Induced Cytidine Deaminase (AID) Deficiency Causes the Autosomal Recessive Form of the Hyper-IgM Syndrome (HIGM2)Cell, 2000
- Evolution of somatic hypermutation and gene conversion in adaptive immunityImmunological Reviews, 1998
- Inducible Site-Directed Recombination in Mouse Embryonic Stem CellsNucleic Acids Research, 1996
- Molecular Cloning of an Apolipoprotein B Messenger RNA Editing ProteinScience, 1993
- RAG-2 expression is not essential for chicken immunoglobulin gene conversion.Proceedings of the National Academy of Sciences of the United States of America, 1992
- Intraclonal generation of antibody mutants in germinal centresNature, 1991
- A hyperconversion mechanism generates the chicken light chain preimmune repertoireCell, 1987
- Evidence for intrachromosomal gene conversion in cultured mouse cellsCell, 1983
- Organization of immunoglobulin heavy chain genes and allelic deletion model.Proceedings of the National Academy of Sciences of the United States of America, 1978