Numbers of Receptor Sites from Scatchard Graphs: Facts and Fantasies
- 24 September 1982
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 217 (4566), 1247-1249
- https://doi.org/10.1126/science.6287580
Abstract
Data for ligand and receptor binding presented in the format of a Scatchard graph are compared with the same data shown as bound ligand plotted against the logarithm of free ligand. From this comparison it is apparent that extrapolations in the Scatchard graph to yield total number of receptor sites are generally not correct.Keywords
This publication has 7 references indexed in Scilit:
- Pentobarbital: Dual Actions to Increase Brain Benzodiazepine Receptor AffinityScience, 1981
- LIGAND: A versatile computerized approach for characterization of ligand-binding systemsAnalytical Biochemistry, 1980
- Direct in vitro effect of a sulfonylurea to increase human fibroblast insulin receptors.JCI Insight, 1980
- Protein affinities for small molecules: Conceptions and misconceptionsArchives of Biochemistry and Biophysics, 1979
- Protein interactions with small moleculesAccounts of Chemical Research, 1974
- Analysis of macromolecule-ligand binding by determination of stepwise equilibrium constantsBiochemistry, 1970
- The Binding of Organic Ions by Proteins1Journal of the American Chemical Society, 1946