Detecting and Understanding the Roles of Nitric Oxide in Biology
Open Access
- 12 July 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Inorganic Chemistry
- Vol. 49 (14), 6338-6348
- https://doi.org/10.1021/ic9022757
Abstract
We are pursuing a dual strategy for investigating the chemistry of nitric oxide as a biological signaling agent. In one approach, metal-based fluorescent sensors for the detection of NO in living cells are evaluated, and a sensor based on a copper fluorescein complex has proved to be a valuable lead compound. Sensors of this class permit identification of NO from both inducible and constitutive forms of nitric oxide synthase and facilitate investigation of different NO functions in response to external stimuli. In the other approach, we employ synthetic model complexes of iron−sulfur clusters to probe their reactivity toward nitric oxide as biomimics of the active sites of iron−sulfur proteins. Our studies reveal that NO disassembles the Fe−S clusters to form dinitrosyl iron complexes.Keywords
This publication has 109 references indexed in Scilit:
- NO formation by a catalytically self-sufficient bacterial nitric oxide synthase from Sorangium cellulosumProceedings of the National Academy of Sciences of the United States of America, 2009
- Analytical Chemistry of Nitric OxideAnnual Review of Analytical Chemistry, 2009
- Dinitrosyl Iron Complexes Relevant to Rieske Cluster NitrosylationJournal of the American Chemical Society, 2009
- Dinitrosyliron complexes and the mechanism(s) of cellular protein nitrosothiol formation from nitric oxideProceedings of the National Academy of Sciences of the United States of America, 2009
- Three-Coordinate Copper(I) Amido and Aminyl Radical ComplexesJournal of the American Chemical Society, 2009
- Reactivity of nitric oxide with the [4Fe–4S] cluster of dihydroxyacid dehydratase fromEscherichia coliBiochemical Journal, 2009
- Nitric Oxide-induced Conversion of Cellular Chelatable Iron into Macromolecule-bound Paramagnetic Dinitrosyliron ComplexesJournal of Biological Chemistry, 2008
- Bacterial Nitric-oxide Synthases Operate without a Dedicated Redox PartnerPublished by Elsevier BV ,2008
- Bacillus anthracis -derived nitric oxide is essential for pathogen virulence and survival in macrophagesProceedings of the National Academy of Sciences, 2008
- Nitrogen monoxide (NO)-mediated iron release from cells is linked to NO-induced glutathione efflux via multidrug resistance-associated protein 1Proceedings of the National Academy of Sciences of the United States of America, 2006