Transcriptional regulation of the redD transcriptional activator gene accounts for growth‐phase‐dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)
- 27 October 1992
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 6 (19), 2797-2804
- https://doi.org/10.1111/j.1365-2958.1992.tb01459.x
Abstract
Transcription of redD, the activator gene required for production of the red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift‐down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth‐phase‐dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters.Keywords
This publication has 33 references indexed in Scilit:
- The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of streptomycesCell, 1991
- Stringent response and initiation of secondary metabolism in Streptomyces clavuligerusJournal of General Microbiology, 1991
- A relaxed (rel) mutant of Streptomyces coelicolor A3(2) with a missing ribosomal protein lacks the ability to accumulate ppGpp, A-factor and prodigiosinJournal of General Microbiology, 1990
- Pigmented antibiotic production by Streptomyces coelicolor A3(2): kinetics and the influence of nutrientsJournal of General Microbiology, 1990
- Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2)Gene, 1990
- Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolorGene, 1988
- The Leeuwenhoek Lecture, 1987 - Towards an understanding of gene switching in Streptomyces , the basis of sporulation and antibiotic productionProceedings of the Royal Society of London. B. Biological Sciences, 1988
- Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicatorMolecular Genetics and Genomics, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequencesNature, 1980