Endometrial regeneration using autologous adult stem cells followed by conception by in vitro fertilization in a patient of severe Asherman′s syndrome
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Open Access
- 1 January 2011
- journal article
- case report
- Published by Medknow in Journal of Human Reproductive Sciences
- Vol. 4 (1), 43-48
- https://doi.org/10.4103/0974-1208.82360
Abstract
In a woman with severe Asherman's syndrome, curettage followed by placement of intrauterine contraceptive device (IUCD) (IUCD with cyclical hormonal therapy) was tried for 6 months, for development of the endometrium. When this failed, autologous stem cells were tried as an alternative therapy. From adult autologous stem cells isolated from patient's own bone marrow, endometrial angiogenic stem cells were separated using immunomagnetic isolation. These cells were placed in the endometrial cavity under ultrasound guidance after curettage. Patient was then given cyclical hormonal therapy. Endometrium was assessed intermittently on ultrasound. On development of endometrium with a thickness of 8 mm and good vascularity, in vitro fertilization and embryo transfer was done. This resulted in positive biochemical pregnancy followed by confirmation of gestational sac, yolk sac, and embryonic pole with cardiac activity on ultrasound. Endometrial angiogenic stem cells isolated from autologous adult stem cells could regenerate injured endometrium not responding to conventional treatment for Asherman's syndrome.Keywords
This publication has 22 references indexed in Scilit:
- Asherman syndrome—one century laterFertility and Sterility, 2008
- Endometrial regenerative cells: A novel stem cell populationJournal of Translational Medicine, 2007
- Contribution of Bone Marrow-Derived Stem Cells to Endometrium and EndometriosisThe International Journal of Cell Cloning, 2007
- Expression of steroid hormone receptors, proliferation and apoptotic markers in primate endometriumMolecular and Cellular Endocrinology, 2006
- Isolation of Renal Progenitor Cells from Adult Human KidneyThe American Journal of Pathology, 2005
- Contribution of bone marrow–derived endothelial cells to human tumor vasculatureNature Medicine, 2005
- Endothelial Progenitor CellsCirculation Research, 2004
- Endometrial Cells Derived From Donor Stem Cells in Bone Marrow Transplant RecipientsJAMA, 2004
- Expression of Novel Surface Antigens on Early Hematopoietic CellsaAnnals of the New York Academy of Sciences, 1999
- CD34+AC133+Cells Isolated from Cord Blood are Highly Enriched in Long‐Term Culture‐Initiating Cells, NOD/SCID‐Repopulating Cells and Dendritic Cell ProgenitorsThe International Journal of Cell Cloning, 1998