Correlated analysis of cellular DNA, membrane antigens and light scatter of human lymphoid cells
Open Access
- 1 March 1982
- Vol. 2 (5), 337-343
- https://doi.org/10.1002/cyto.990020511
Abstract
Flow cytometric correlated analysis of membrane antigens, DNA, and light scatter was performed on human lymphoid cells using fluorescein (FITC)-conjugated antibodies to label B- and T-cell antigens and propidium iodide (PI) to stain DNA after ethanol fixation and RNase treatment. A FACS II flow cytometer was modified to obtain digitized measurements of two color fluorescence and light scatter emissions, simultaneously. Software was written to allow single parameter analysis or correlated analysis of any two of the three parameters acquired. Ethanol fixation preserved FITC surface labeling for at least 15 weeks, but produced marked changes in light scatter. No changes in FITC distributions were observed after RNase treatment and PI staining, and the presence of FITC labeling did not affect DNA distributions. Within heterogeneous cell populations, the DNA distribution of cell subpopulations identified by a membrane antigen was clearly demonstrated.Keywords
This publication has 12 references indexed in Scilit:
- Studies of a human T lymphocyte antigen recognized by a monoclonal antibody.Proceedings of the National Academy of Sciences of the United States of America, 1981
- Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry.The Journal of Immunology, 1981
- Simultaneous quantitation of hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorterCytometry, 1980
- Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood.Proceedings of the National Academy of Sciences of the United States of America, 1980
- Cellular DNA content as a marker of neoplasia in manAmerican Journal Of Medicine, 1980
- Analysis and sorting of living cells according to deoxyribonucleic acid content.Journal of Histochemistry & Cytochemistry, 1977
- Fluorescence-activated cell sorting of human T and B lymphocytes: I. Direct evidence that lymphocytes with a high density of membrane-bound immunoglobulin are precursors of plasmacytesCellular Immunology, 1974
- Expression of membrane‐bound IgM and HL-A antigens on lymphoblastoid cells in different stages of the cell cycleEuropean Journal of Immunology, 1974
- Separation of Viable from Non-Viable Tumor Cells Using Ficoll-Hypaque Density SolutionImmunological Communications, 1973
- THE USE OF AN ETHIDIUM ANALOGUE IN THE DYE-BUOYANT DENSITY PROCEDURE FOR THE ISOLATION OF CLOSED CIRCULAR DNA: THE VARIATION OF THE SUPERHELIX DENSITY OF MITOCHONDRIAL DNAProceedings of the National Academy of Sciences of the United States of America, 1969