The probioticEscherichia colistrain Nissle 1917 interferes with invasion of human intestinal epithelial cells by different enteroinvasive bacterial pathogens
Open Access
- 1 April 2004
- journal article
- Published by Oxford University Press (OUP) in FEMS Immunology & Medical Microbiology
- Vol. 40 (3), 223-229
- https://doi.org/10.1016/s0928-8244(03)00368-7
Abstract
The probiotic Escherichia coli strain Nissle 1917 (Mutaflor®) of serotype O6:K5:H1 was reported to protect gnotobiotic piglets from infection with Salmonella enterica serovar Typhimurium. An important virulence property of Salmonella is invasion of host epithelial cells. Therefore, we tested for interference of E. coli strain Nissle 1917 with Salmonella invasion of INT407 cells. Simultaneous administration of E. coli strain Nissle 1917 and Salmonella resulted in up to 70% reduction of Salmonella invasion efficiency. Furthermore, invasion of Yersinia enterocolitica, Shigella flexneri, Legionella pneumophila and even of Listeria monocytogenes were inhibited by the probiotic E. coli strain Nissle 1917 without affecting the viability of the invasive bacteria. The observed inhibition of invasion was not due to the production of microcins by the Nissle 1917 strain because its isogenic microcin-negative mutant SK22D was as effective as the parent strain. Reduced invasion rates were also achieved if strain Nissle 1917 was separated from the invasive bacteria as well as from the INT407 monolayer by a membrane non-permeable for bacteria. We conclude E. coli Nissle 1917 to interfere with bacterial invasion of INT407 cells via a secreted component and not relying on direct physical contact with either the invasive bacteria or the epithelial cells.Keywords
This publication has 28 references indexed in Scilit:
- A Single Nucleotide Exchange in the wzy Gene Is Responsible for the Semirough O6 Lipopolysaccharide Phenotype and Serum Sensitivity of Escherichia coli Strain Nissle 1917Journal of Bacteriology, 2002
- Gut bacteria and health foods—the European perspectiveInternational Journal of Food Microbiology, 2002
- Can bacterial interference prevent infection?Trends in Microbiology, 2001
- Non-pathogenic Escherichia coli versus mesalazine for the treatment of ulcerative colitis: a randomised trialThe Lancet, 1999
- Effect of Preventive Administration of a Nonpathogenic Escherichia coli Strain on the Colonization of the Intestine with Microbial Pathogens in Newborn InfantsNeonatology, 1997
- Properties ofEscherichia coli strains of serotype O6Infection, 1995
- Genetics of bacteriocins produced by lactic acid bacteriaFEMS Microbiology Reviews, 1993
- The Antibody Response in Breast-Fed and Non-Breast-Fed Infants after Artificial Colonization of the Intestine with Escherichia coli 083Pediatric Research, 1991
- Bacteriocins of lactic acid bacteriaBiochimie, 1988
- The microcinsFEMS Microbiology Letters, 1984