T regulatory cells and Th1/Th2 cytokines in peripheral blood from tuberculosis patients
- 21 March 2010
- journal article
- Published by Springer Science and Business Media LLC in European Journal of Clinical Microbiology & Infectious Diseases
- Vol. 29 (6), 643-650
- https://doi.org/10.1007/s10096-010-0908-0
Abstract
About 10% of people infected with Mycobacterium tuberculosis develop active tuberculosis (TB), and Th1 effector cells and Th1 cytokines play key roles in controlling M. tuberculosis infection. Here, we hypothesise that this susceptibility to M. tuberculosis infection is linked to increased T regulatory (Treg) cells and Th2 cytokines in TB patients. To test this, we recruited 101 participants (71 TB patients, 12 non-TB pulmonary diseases and 18 healthy subjects) and investigated Treg cells and Th1/Th2 cytokines in peripheral blood. CD4+CD25+ T cells and CD4+CD25+FoxP3+ T cells significantly increased and IL-5 dramatically decreased in TB patients relative to healthy subjects. CD8+CD28− T cells, IFN-γ, TNF-α, IL-10 and IL-4 significantly increased in patients with culture and sputum smear-positive pulmonary TB (PTB(+)) compared with healthy subjects. CD4+CD25+FoxP3+ and CD8+CD28− T cells significantly decreased in PTB(+) after one month of chemotherapy. CD4+, CD4+CD25+ and CD8+CD28+ T cells significantly increased in extra-pulmonary TB patients after one month of chemotherapy. These findings suggest that M. tuberculosis infection induces circulating CD4+CD25+FoxP3+ and CD8+CD28− T cell expansion, which may be related to the progression of M. tuberculosis infection, and that the balance between effector immune responses and suppression immune responses is essential to control M. tuberculosis infection.Keywords
This publication has 26 references indexed in Scilit:
- Characterization of regulatory T cells identified as CD4+CD25highCD39+ in patients with active tuberculosisClinical and Experimental Immunology, 2009
- Inhibitory CD8+ T cells in autoimmune diseaseHuman Immunology, 2008
- CD8+CD28−, suppressive T cells in systemic lupus erythematosusLupus, 2008
- In vitro expansion of CD4+CD25highFOXP3+CD127low/− regulatory T cells from peripheral blood lymphocytes of healthy Mycobacterium tuberculosis-infected humansMicrobes and Infection, 2007
- Expansion and function of Foxp3-expressing T regulatory cells during tuberculosisThe Journal of Experimental Medicine, 2007
- FOXP3 gene expression in a tuberculosis case contact studyClinical and Experimental Immunology, 2007
- Immunosuppression during Active Tuberculosis Is Characterized by Decreased Interferon‐γ Production and CD25 Expression with Elevated Forkhead Box P3, Transforming Growth Factor–β, and Interleukin‐4 mRNA LevelsThe Journal of Infectious Diseases, 2007
- Inactivation of CD4+CD25+ regulatory T cells during early mycobacterial infection increases cytokine production but does not affect pathogen loadImmunology & Cell Biology, 2006
- The Immune Response to BCG Vaccination of NewbornsAnnals of the New York Academy of Sciences, 2005
- Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell levelEuropean Journal of Immunology, 2005