Proteome Profiling of Populus euphratica Oliv. Upon Heat Stress
Open Access
- 1 June 2006
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of Botany
- Vol. 98 (2), 361-377
- https://doi.org/10.1093/aob/mcl106
Abstract
• Background and Aims Populus euphratica is a light-demanding species ecologically characterized as a pioneer. It grows in shelter belts along riversides, being part of the natural desert forest ecosystems in China and Middle Eastern countries. It is able to survive extreme temperatures, drought and salt stress, marking itself out as an important plant species to study the mechanisms responsible for survival of woody plants under heat stress. • Methods Heat effects were evaluated through electrolyte leakage on leaf discs, and LT50 was determined to occur above 50 °C. Protein accumulation profiles of leaves from young plants submitted to 42/37 °C for 3 d in a phytotron were determined through 2D-PAGE, and a total of 45 % of up- and downregulated proteins were detected. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)/TOF analysis, combined with searches in different databases, enabled the identification of 82 % of the selected spots. • Key Results Short-term upregulated proteins are related to membrane destabilization and cytoskeleton restructuring, sulfur assimilation, thiamine and hydrophobic amino acid biosynthesis, and protein stability. Long-term upregulated proteins are involved in redox homeostasis and photosynthesis. Late downregulated proteins are involved mainly in carbon metabolism. • Conclusions Moderate heat response involves proteins related to lipid biogenesis, cytoskeleton structure, sulfate assimilation, thiamine and hydrophobic amino acid biosynthesis, and nuclear transport. Photostasis is achieved through carbon metabolism adjustment, a decrease of photosystem II (PSII) abundance and an increase of PSI contribution to photosynthetic linear electron flow. Thioredoxin h may have a special role in this process in P. euphratica upon moderate heat exposure.Keywords
This publication has 81 references indexed in Scilit:
- Tomato Heat Stress Transcription Factor HsfB1 Represents a Novel Type of General Transcription Coactivator with a Histone-Like Motif Interacting with the Plant CREB Binding Protein Ortholog HAC1[W]THE PLANT CELL ONLINE, 2004
- Emerging Use of Gene Expression Microarrays in Plant PhysiologyComparative and Functional Genomics, 2003
- Dual action of the active oxygen species during plant stress responsesCellular and Molecular Life Sciences, 2000
- Actin cytoskeleton in plants: From transport networks to signaling networksMicroscopy Research and Technique, 1999
- Beyond Nuclear TransportThe Journal of cell biology, 1999
- Nucleocytoplasmic trafficking of proteins: With or without Ran?BioEssays, 1999
- Proteolysis of the phage λ CII regulatory protein by FtsH (HflB) of Escherichia coliMolecular Microbiology, 1997
- Photosynthetic response of Populus euphratica to salt stressForest Ecology and Management, 1997
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- The PSI‐E subunit of photosystem I binds ferredoxin:NADP+ oxidoreductaseFEBS Letters, 1992