A rapid and sensitive bioassay to measure bone morphogenetic protein activity
Open Access
- 19 September 2007
- journal article
- Published by Springer Science and Business Media LLC in BMC Cell Biology
- Vol. 8 (1), 41
- https://doi.org/10.1186/1471-2121-8-41
Abstract
Bone morphogenetic proteins (BMPs) are members of the TGF-beta superfamily and were originally identified as proteins that induce ectopic bone formation. BMPs were shown subsequently to be involved in several biological processes during development and in adult tissues through the regulation of the growth, differentiation and apoptosis of various cell types. An alkaline phosphatase (ALP)-based assay is the most widely used assay to evaluate BMP activity. However, the ALP assay is not rapid and not sensitive enough to measure BMP activity at physiological concentrations. In this paper, we describe a highly sensitive, rapid, and specific cell-based assay for the quantification of BMP activity.Keywords
This publication has 37 references indexed in Scilit:
- Bone morphogenetic protein antagonist gremlin 1 is widely expressed by cancer-associated stromal cells and can promote tumor cell proliferationProceedings of the National Academy of Sciences of the United States of America, 2006
- Modulator of bone morphogenetic protein activity in the progression of kidney diseasesKidney International, 2006
- Bone Morphogenetic Protein-6 Promotes Osteoblastic Prostate Cancer Bone Metastases through a Dual MechanismCancer Research, 2005
- Increased Susceptibility to Pulmonary Hypertension in Heterozygous BMPR2-Mutant MiceCirculation, 2005
- Disruption of LTBP-4 function reduces TGF-β activation and enhances BMP-4 signaling in the lungThe Journal of cell biology, 2004
- Cbl-mediated Degradation of Lyn and Fyn Induced by Constitutive Fibroblast Growth Factor Receptor-2 Activation Supports Osteoblast DifferentiationPublished by Elsevier BV ,2004
- Mechanisms of TGF-β Signaling from Cell Membrane to the NucleusCell, 2003
- Cerebrospinal Fluid Contains Biologically Active Bone Morphogenetic Protein-7Experimental Neurology, 2001
- Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]The Journal of cell biology, 1994
- Novel Regulators of Bone Formation: Molecular Clones and ActivitiesScience, 1988