Anti-inflammatory effects of tectroside on UVB-induced HaCaT cells
- 11 April 2013
- journal article
- Published by Spandidos Publications in International Journal of Molecular Medicine
- Vol. 31 (6), 1471-1476
- https://doi.org/10.3892/ijmm.2013.1343
Abstract
Ultraviolet B (UVB) irradiation causes skin damage and inflammation by inducing the secretion of various cytokines, which are immune regulators produced by cells. To prevent skin inflammation, keratinocytes that have been irreversibly damaged by UVB must be eliminated through apoptosis. Ixeris dentata (I. dentata) (family Asteraceae) is a perennial medicinal herb indigenous to Korea. It is used in Korea, China and Japan to treat indigestion, pneumonia, diabetes, hepatitis, contusions and tumors. Guaiane-type sesquiterpene lactones were isolated from the whole extract of I. dentata. This led to the isolation of the anti-inflammatory sesquiterpene lactone compound tectroside (TES), which was tested on a human keratinocyte cell line. To determine the anti-inflammatory effects of TES, we examined its influence on UVB-induced pro-inflammatory cytokine production in human keratinocytes (HaCaT cells) by observing these cells in the presence or absence of TES. In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction and western blot analysis to evaluate the activation of mitogen-activated protein kinases (MAPKs). TES inhibited UVB-induced production of the pro-inflammatory cytokines interleukin (IL)-6 and IL-8 in a dose-dependent manner. In addition, TES inhibited the expression of cyclooxygenase (COX)-2 and the phosphorylation of c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) MAPKs, suggesting that it inhibits the secretion of the pro-inflammatory cytokines IL-6 and IL-8 and COX-2 expression by blocking MAPK phosphorylation. These results suggest that TES can potentially protect against UVB-induced skin inflammation.Keywords
This publication has 24 references indexed in Scilit:
- Effects of Ixeris dentata ext. on Lowering Lipid and Anti-oxidationKorean Journal of Plant Resources, 2011
- Photoageing: mechanism, prevention and therapyBritish Journal of Dermatology, 2007
- Photochemoprevention of ultraviolet B signaling and photocarcinogenesisMutation research. Reviews in mutation research, 2005
- Ultraviolet B-induced cell death in four cutaneous cell lines exhibiting different enzymatic antioxidant defences: involvement of apoptosisJournal of Dermatological Science, 1998
- Ultraviolet Light and Osmotic Stress: Activation of the JNK Cascade Through Multiple Growth Factor and Cytokine ReceptorsScience, 1996
- Preprogrammed and Programmed Cell Death Mechanisms of Apoptosis: UV‐Induced Immediate and Delayed ApoptosisPhotochemistry and Photobiology, 1996
- Both UVA and UVB induce cytoskeleton-dependent surface blebbing in epidermoid cellsJournal of Photochemistry and Photobiology B: Biology, 1994
- Disruption of cytoplasmic microtubules by ultraviolet radiationExperimental Cell Research, 1991
- RESEARCH NOTEPhotochemistry and Photobiology, 1990
- Environmental Wavelengths of Ultraviolet Light Induce Cytoskeletal DamageJournal of Investigative Dermatology, 1987