Nano-manipulation of actomyosin molecular motors in vitro: a new working principle
- 30 September 1993
- journal article
- review article
- Published by Elsevier BV in Trends in Biochemical Sciences
- Vol. 18 (9), 319-324
- https://doi.org/10.1016/0968-0004(93)90064-t
Abstract
Techniques have been recently developed that allow the direct observation of single actin filaments and their manipulation, using glass microneedles, in the nanometer range. Further development of these techniques has made possible the detection of subpiconewton-level forces of individual myosin heads. This in vitro motility model is sensitive in the submillisecond range and has allowed us to determine the force generation of an actomyosin motor directly at the molecular level. The results have led to a new conceptual framework for chemo-mechanical energy transduction in the molecular motor.Keywords
This publication has 22 references indexed in Scilit:
- Sub-piconewton force fluctuations of actomyosin in vitroNature, 1991
- Force measurements by micromanipulation of a single actin filament by glass needlesNature, 1988
- Direct observation of molecular motility by light microscopyCell Motility, 1988
- Myosin subfragment-1 is sufficient to move actin filaments in vitroNature, 1987
- Direct observation of motion of single F-actin filaments in the presence of myosinNature, 1984
- Video image processing greatly enhances contrast, quality, and speed in polarization-based microscopy.The Journal of cell biology, 1981
- Visualization of Individual DNA Molecules in Solution by Light Microscopy: DAPI Staining Method1The Journal of Biochemistry, 1981
- Amatoxins, Phallotoxins, Phallolysin, and Antamanide: The Biologically Active Components of PoisonousAmanitaMushroomCRC Critical Reviews in Biochemistry, 1978
- Fluctuations in tension during contraction of single muscle fibersBiophysical Journal, 1977
- Light microscope study of mixed helices in reconstituted Salmonella flagellaJournal of Molecular Biology, 1976