The synthesis of polyphenylalanine on ribosomes to which erythromycin is bound
- 1 June 1991
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 198 (3), 713-722
- https://doi.org/10.1111/j.1432-1033.1991.tb16071.x
Abstract
Erythromycin binds to the large subunit of Escherichia coli ribosomes at a specific site that is very close to the amino acid of aminoacyl-tRNA bound into the peptidyltransferase center, and to the site to which puromycin is bound, the P and A sites, respectively, of the classical two-site model of ribosome function. Both erythromycin and puromycin affect fluorescence from fluorescent derivatives of aminoacyl-tRNAs, while both puromycin and aminoacyl-tRNAs affect fluorescence of fluorescent derivatives of erythromycylamine. The results demonstrate unequivocally that erythromycin, deacylated tRNA, a peptidyl-tRNA analogue and puromycin can be bound simultaneously to the same ribosome. Nascent peptides of more than a few amino acids in length block binding of erythromycin to the ribosomes but, unlike most other peptides, long polyphenylalanine chains can be synthesized on ribosomes to which erythromycin is bound. It is suggested that this refractory synthesis in the presence of erythromycin reflects the atypical physical and structural properties of polyphenylalanine.Keywords
This publication has 34 references indexed in Scilit:
- Movement of tRNA but not the nascent peptide during peptide bond formation on ribosomesBiochemistry, 1990
- An apparent conformational change in tRNAPhe that is associated with the peptidyl transferase reactionBiochimie, 1987
- Localization of L11 on the Escherichia coli ribosome by singlet-singlet energy transferEuropean Journal of Biochemistry, 1986
- Stereochemical analysis of ribosomal transpeptidation conformation of nascent peptideJournal of Molecular Biology, 1986
- Binding of S21 to the 50S subunit and the effect of the 50S subunit on nonradiative energy transfer between the 3' end of 16S RNA and S21Biochemistry, 1984
- Localization of the elongation factor EF-Tu binding site on Escherichia coli ribosomesBiochemistry, 1983
- Position of transfer ribonucleic acid on Escherichia coli ribosomes. Distance from the 3' end of 16S ribonucleic acid to three points on phenylalanine-accepting transfer ribonucleic acid in the donor site of 70S ribosomesBiochemistry, 1981
- Distances between 3' ends of ribosomal ribonucleic acids reassembled into Escherichia coli ribosomesBiochemistry, 1980
- Globin mRNA Translation on Artemia salina Ribosomes with Components from Friend Leukemia CellsEuropean Journal of Biochemistry, 1975
- Erythromycin, a peptidyltransferase effectorBiochemistry, 1972