Isolation and Characterization of Listeria monocytogenes Isolates from Ready-To-Eat Foods in Florida

Abstract

Of 3,063 ready-to-eat food samples tested, 91 (2.97%) were positive for Listeria monocytogenes , and lineage 1 strains outnumbered lineage 2 strains 57 to 34. Seventy-one isolates (78%) exhibited multiple antibiotic resistance, and an L. monocytogenes -specific bacteriophage cocktail lysed 65 of 91 (71%) isolates. Determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods.