Design and evaluation of genome-wide libraries for RNA interference screens
Open Access
- 1 January 2010
- journal article
- Published by Springer Science and Business Media LLC in Genome Biology
- Vol. 11 (6), R61
- https://doi.org/10.1186/gb-2010-11-6-r61
Abstract
RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/.Keywords
This publication has 55 references indexed in Scilit:
- The art and design of genetic screens: RNA interferenceNature Reviews Genetics, 2008
- Specialization and evolution of endogenous small RNA pathwaysNature Reviews Genetics, 2007
- A genome-wide transgenic RNAi library for conditional gene inactivation in DrosophilaNature, 2007
- Cellular phenotyping by RNAiBriefings in Functional Genomics and Proteomics, 2006
- An algorithm for selection of functional siRNA sequencesBiochemical and Biophysical Research Communications, 2004
- Rational siRNA design for RNA interferenceNature Biotechnology, 2004
- Functional siRNAs and miRNAs Exhibit Strand BiasCell, 2003
- Asymmetry in the Assembly of the RNAi Enzyme ComplexCell, 2003
- RNAi in Human Cells: Basic Structural and Functional Features of Small Interfering RNAMolecular Cell, 2002
- Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegansNature, 1998