Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing
- 1 October 1993
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 365 (6447), 671-673
- https://doi.org/10.1038/365671a0
Abstract
A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.Keywords
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