Increasing anthropogenic pressure, global climate change, and the lack of large introduction centers in the Chernihiv region makes it important to preserve rare species of flora ex situ outside this administrative region. The article presents the results of the study of taxonomic composition and evaluation of the success of the introduction of rare plant species of Chernihiv region in the M. M. Gryshko National Botanical Garden of National Academy of Sciences of Ukraine. The study of rare plant species and the development of methods for their effective reproduction was initiated in the NBG in 1970 in a separate section “Rare plants of the flora of Ukraine.” It is established that the collection grows and protects 57 phythorarites of Chernihiv region, of which 29 species are listed in the Red Book of Ukraine (III edition), and 28 species – in the “List of regionally rare plant species of Chernihiv region”. Most plants successfully recover ex situ with moderate care or without additional human intervention. The biomorphological spectrum of introduced plants is dominated by cryptophytes (50.88 %) and hemicryptophytes (42.11 %), the shares of phanerophytes, hamephytes and therophytes are insignificant. 17 species of phythorarites formed stable homeostatic populations. Of these: 5 species are listed in the Red Book of Ukraine (Allium ursinum, Crocus reticulatus, Epipactis helleborine, Galanthus nivalis, Pulsatilla pratensis) and 12 species – in the “List of regionally rare plant species of Chernihiv region” (Aster amellus, Corydalis intermedia, C. marschal, Daphne mesereum, Equisetum hyemale, Iris hungarica, Phlomis tuberosa, Primula veris, Pteridium aquilinum, Scilla bifolia, S. sibirica, Vinca minor). Thus, the cultivation of almost a third of the phythorarites of Chernihiv region in the M. M. Gryshko National Botanical Garden testifies to the effectiveness of their preservation ex situ.