Isolation of Human and Mouse Hematopoietic Stem Cells
- 1 January 2009
- book chapter
- Published by Springer Science and Business Media LLC in Methods in molecular biology (Clifton, N.J.)
- Vol. 506, 13-21
- https://doi.org/10.1007/978-1-59745-409-4_2
Abstract
Hematopoietic stem cells (HSC) are rare with estimated frequencies of 1 in 10,000 bone marrow cells and 1 in every 100,000 blood cells. The most important characteristic of HSC is their capacity to provide complete restoration of all blood cell lineages after bone marrow ablation. Therefore they are considered as the ideal targets for various clinical applications including stem cell transplantation and gene therapy. In adult mice and men, the main stem cell source is the bone marrow. For clinical applications HSC derived from umbilical cord blood (UCB) and G-CSF mobilized peripheral blood (PB) have been demonstrated to have several advantages compared to bone marrow; therefore, they are slowly replacing BM as alternative source of stem cells. The mouse is the model organism of choice for immunological and hematological research; therefore, studies of murine HSC are an important research topic. Here we described the most often used protocols and methods to isolate human and mouse HSC to high purity.This publication has 8 references indexed in Scilit:
- Ectopic retroviral expression of LMO2, but not IL2Rγ, blocks human T-cell development from CD34+ cells: implications for leukemogenesis in gene therapyLeukemia, 2007
- Hematopoietic Stem Cell Antigen CD34: Role in Adhesion or HomingStem Cells and Development, 2006
- Identification of hematopoietic stem/progenitor cells: strength and drawbacks of functional assaysOncogene, 2004
- Gene-expression profiling of CD34+ cells from various hematopoietic stem-cell sources reveals functional differences in stem-cell activityJournal of Leukocyte Biology, 2003
- Biology of Hematopoietic Stem Cells and Progenitors: Implications for Clinical ApplicationAnnual Review of Immunology, 2003
- Selectivein vitroexpansion and efficient retroviral transduction of human CD34+CD38-haematopoietic stem cellsBritish Journal of Haematology, 2002
- Purified hematopoietic stem cells can differentiate into hepatocytes in vivoNature Medicine, 2000