Mutations in therpsLGene Are Involved in Streptomycin Resistance inCampylobacter coli
- 1 June 2010
- journal article
- research article
- Published by Mary Ann Liebert Inc in Microbial Drug Resistance
- Vol. 16 (2), 105-110
- https://doi.org/10.1089/mdr.2009.0128
Abstract
To characterize the mechanisms of streptomycin (STR) resistance in Campylobacter coli, we chose 17 isolates that were resistant to STR, erythromycin (ERY), or both, and the putative STR resistance target genes rpsL, rrs, and gidB were analyzed for mutations. The presence of the aadE gene encoding aminoglycoside 6-adenylyltransferase was also evaluated. To reveal putative connection between ERY and STR resistance mechanisms, 13 C. coli isolates initially susceptible to STR and ERY were exposed to STR, and resistant variants were characterized. We also assessed the development of ERY resistance with a similar method. Finally, the effect of the putative CmeABC efflux pump inhibitor phenyl-arginine-β-naphthylamine on STR resistance was tested. Our studies showed an association between mutations in the rpsL gene and STR resistance in C. coli. Further, mutations obtained in vitro were more diverse than those occurring in vivo. However, we observed no resistance associated mutations in the other genes studied, and selection with STR did not result in variants resistant to ERY and vice versa. None of the isolates harbored the aadE gene, and no differences in STR minimum inhibitory concentration levels were detected in the presence or absence of phenyl-arginine-β-naphthylamine. In conclusion, we found that STR resistance was associated with mutations in the rpsL gene, but no obvious association between STR and ERY resistance mechanisms was found in C. coli.Keywords
This publication has 25 references indexed in Scilit:
- Identification of Mutations Related to Streptomycin Resistance in Clinical Isolates of Mycobacterium tuberculosis and Possible Involvement of Efflux MechanismAntimicrobial Agents and Chemotherapy, 2008
- Effect of putative efflux pump inhibitors and inducers on the antimicrobial susceptibility of Campylobacter jejuni and Campylobacter coliJournal of Medical Microbiology, 2008
- Contribution of the CmeABC Efflux Pump to Macrolide and Tetracycline Resistance in Campylobacter jejuniAntimicrobial Agents and Chemotherapy, 2007
- Mosaic Structure of a Multiple-Drug-Resistant, Conjugative Plasmid from Campylobacter jejuniAntimicrobial Agents and Chemotherapy, 2005
- Major Structural Differences and Novel Potential Virulence Mechanisms from the Genomes of Multiple Campylobacter SpeciesPLoS Biology, 2005
- Characterization of Plasmid-MediatedaphA-3Kanamycin Resistance inCampylobacter jejuniMicrobial Drug Resistance, 2004
- The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coliMolecular Genetics and Genomics, 2004
- Antimicrobial resistance of thermophilic CampylobacterVeterinary Research, 2001
- Rapid extraction of bacterial genomic DNA with guanidium thiocyanateLetters in Applied Microbiology, 1989
- Ribosomal proteins: XXXIII. Location of amino-acid replacements in protein S12 isolated from Escherichia coli mutants resistant to streptomycinJournal of Molecular Biology, 1972