SUBCELLULAR LOCALIZATION OF HEMATOPORPHYRIN DERIVATIVE IN BLADDER TUMOR CELLS IN CULTURE
- 1 April 1990
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 51 (4), 451-457
- https://doi.org/10.1111/j.1751-1097.1990.tb01736.x
Abstract
— Mitochondria have been implicated as a primary subcellular site of porphyrin localization and photodestruction. However, other organelles including the cell membrane, lysosomes and nucleus have been shown to be damaged by hematoporphyrin derivative (HpD) photosensitized destruction as well. In this study we attempted to follow the translocation of the fluorescent components of HpD in human bladder tumor cells (MGH-U1) in culture to determine whether specific subcellular localization occurs over time. Following a 30 min exposure to HpD the cellular fluorescence was examined immediately and 1, 2, 4, and 24 h after HpD removal using fluorescence microscopy and an interactive laser cytometer. The in vitro translocation of dye appeared to be fairly rapid with fluorescence present at the cell membrane and later (1-2 h) within a perinuclear area of the cytoplasm. To determine whether HpD had become concentrated into a specific subcellular organelle, these fluorescence distribution patterns were compared with fluorescent marker dyes specific for mitochondria, endoplasmic reticulum and other membranous organelles. The HpD fluorescence did not appear to be as discrete as the dyes specific for mitochondria or endoplasmic reticulum but appeared similar to the diffuse cytomembrane stain. Finally, the interaction between the fluorescent components of HpD and the cellular constituents was evaluated using a “fluorescence redistribution after photobleaching” technique. The results indicated that the mean lateral diffusion for HpD in MGH-U1 cells was 1.05 times 10−8 cm2/s, a rate closer to that of lipid diffusion (10 K) than that of protein diffusion (10−10). Under our experimental conditions, these studies indicated that upon entering the cell the fluorescent components of HpD rapidly translocated to lipophilic cytomembranous structures but did not appear to partition into a specific subcellular site.This publication has 16 references indexed in Scilit:
- Defining Lipid Transport Pathways in Animal CellsScience, 1985
- Photodynamic inactivation of selected intracellular enzymes by hematoporphyrin derivative and their relationship to tumor cell viability in vitroCancer Letters, 1984
- Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyesCell, 1984
- Cellular uptake of hematoporphyrin derivative in KK-47 bladder cancer cellsUrological Research, 1984
- Distribution of endogenous and injected porphyrins at the subcellular level in rat hepatocytes and in ascites hepatomaChemico-Biological Interactions, 1981
- Photodynamic Effects of Haematoporphyrin on Respiration and Calcium Uptake in Isolated MitochondriaInternational Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1981
- Localization of mitochondria in living cells with rhodamine 123.Proceedings of the National Academy of Sciences of the United States of America, 1980
- Effects of photoactivated porphyrins at the cell surface of leukemia L1210 cellsBiochemistry, 1977
- Photodynamic action of protoporphyrin IX on human erythrocytes: Cross-linking of membrane proteinsBiochemical and Biophysical Research Communications, 1976
- PRIMARY MECHANISMS OF ERYTHROCYTE PHOTOLYSIS INDUCED BY BIOLOGICAL SENSITIZERS AND PHOTOTOXIC DRUGSPhotochemistry and Photobiology, 1975