Cell Uptake and in Vitro Toxicity of Magnetic Nanoparticles Suitable for Drug Delivery
- 26 May 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Molecular Pharmaceutics
- Vol. 6 (5), 1417-1428
- https://doi.org/10.1021/mp900083m
Abstract
Magnetic targeting is useful for intravascular or intracavitary drug delivery, including tumor chemotherapy or intraocular antiangiogenic therapy. For all such in vivo applications, the magnetic drug carrier must be biocompatible and nontoxic. In this work, we investigated the toxic properties of magnetic nanoparticles coated with polyethylenoxide (PEO) triblock copolymers. Such coatings prevent the aggregation of magnetic nanoparticles and guarantee consistent magnetic and nonmagnetic flow properties. It was found that the PEO tail block length inversely correlates with toxicity. The nanoparticles with the shortest 0.75 kDa PEO tails were the most toxic, while particles coated with the 15 kDa PEO tail block copolymers were the least toxic. Toxicity responses of the tested prostate cancer cell lines (PC3 and C4-2), human umbilical vein endothelial cells (HUVECs), and human retinal pigment epithelial cells (HRPEs) were similar. Furthermore, all cell types took up the coated magnetic nanoparticles. It is concluded that magnetite nanoparticles coated with triblock copolymers containing PEO tail lengths of above 2 kDa are biocompatible and appropriate for in vivo application.Keywords
This publication has 60 references indexed in Scilit:
- Stem cell therapy: MRI guidance and monitoringJournal of Magnetic Resonance Imaging, 2008
- Targeted delivery of magnetic aerosol droplets to the lungNature Nanotechnology, 2007
- Magnetic and fluorescent nanoparticles for multimodality imagingNanomedicine, 2007
- Contrast agents for hepatic MRICancer Imaging, 2007
- Factors Affecting Magnetic Retention of Particles in the Upper Airways: An In Vitro and Ex Vivo StudyJournal of Aerosol Medicine, 2006
- Superparamagnetic iron oxide contrast agents: physicochemical characteristics and applications in MR imagingEuropean Radiology, 2001
- Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cellsNature Biotechnology, 2000
- In vivo magnetic resonance imaging of transgene expressionNature Medicine, 2000
- Targeting and retention of magnetic targeted carriers (MTCs) enhancing intra-arterial chemotherapyJournal of Magnetism and Magnetic Materials, 1999
- Biomedical applications of magnetic fluids. I. Magnetic guidance of ferro-colloid-entrapped albumin microsphere for site specific drug delivery in vivo.Journal of Pharmacobio-Dynamics, 1980