Accumulation of Glucose 6-Phosphate or Fructose 6-Phosphate Is Responsible for Destabilization of Glucose Transporter mRNA inEscherichia coli
Open Access
- 1 May 2003
- journal article
- Published by Elsevier BV
- Vol. 278 (18), 15608-15614
- https://doi.org/10.1074/jbc.m300177200
Abstract
Previously we found that a mutation in either pgi or pfkA, encoding phosphoglucose isomerase or phosphofructokinase A, respectively, facilitates degradation of the ptsG mRNA in an RNase E-dependent manner in Escherichia coli (1). In this study, we examined the effects of a series of glycolytic genes on the degradation of ptsG mRNA and how the mutations destabilize the ptsG mRNA. The conditional lethal mutation ts8 in fda, encoding fructose-1,6-P2 aldolase just downstream ofpfkA in the glycolytic pathway, caused the destabilization of ptsG mRNA at the nonpermissive temperature. Mutations in any other gene did not destabilize the ptsGmRNA; rather, they reduced the ptsG transcription mainly by affecting the cAMP level. The rapid degradation ofptsG mRNA in mutant strains was completely dependent upon the presence of glucose or any one of its compounds, which enter the Embden-Meyerhof glycolytic pathway before the block points. A significant increase in the intracellular glucose-6-P level was observed in the presence of glucose in the pgi strain. An overexpression of glucose-6-phosphate dehydrogenase eliminated both the accumulation and the degradation of ptsG mRNA in the pgi strain. In addition, accumulation of fructose-6-P led to the rapid degradation of ptsG mRNA in a pgi pfkA mutant strain lacking glucose-6-P. We conclude that the RNase E-dependent destabilization ofptsG mRNA occurs in response to accumulation of glucose-6-P or fructose-6-P.Keywords
This publication has 36 references indexed in Scilit:
- mRNA Decay inEscherichia coliComes of AgeJournal of Bacteriology, 2002
- HfqMolecular Cell, 2002
- RNase E: still a wonderfully mysterious enzymeMolecular Microbiology, 1997
- Functional relationship between Escherichia coli RNase E and the CafA proteinMolecular Genetics and Genomics, 1997
- Down regulation of cAMP production by cAMP receptor protein in Escherichia coli: an assessment of the contributions of transcriptional and posttranscriptional control of adenylate cyclaseMolecular Genetics and Genomics, 1996
- A DEAD-box RNA helicase in the Escherichia coli RNA degradosomeNature, 1996
- Mechanism responsible for glucose–lactose diauxie in Escherichia coli: challenge to the cAMP modelGenes to Cells, 1996
- A protein complex mediating mRNA degradation in Escherichia coliMolecular Microbiology, 1994
- A lowered concentration of cAMP receptor protein caused by glucose is an important determinant for catabolite repression in Escherichia coliMolecular Microbiology, 1993
- Cyclic AMP regulates Catabolite and Transient Repression in E. coliNature, 1969