Characterization of plasmids harbouring qnrS1, qnrB2 and qnrB19 genes in Salmonella
Open Access
- 11 November 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 63 (2), 274-281
- https://doi.org/10.1093/jac/dkn470
Abstract
The aim of this study was to identify and characterize plasmids carrying qnrS1, qnrB2 and qnrB19 genes identified in Salmonella strains from The Netherlands. The identification of plasmids may help to follow the dissemination of these resistance genes in different countries and environments. Plasmids from 33 qnr-positive Salmonella strains were transferred to Escherichia coli and analysed by restriction, Southern blot hybridization, PCR and sequencing of resistance determinants. They were also assigned to incompatibility groups by PCR-based replicon typing, including three additional PCR assays for the IncU, IncR and ColE groups. The collection included isolates from humans and one from chicken meat. Five IncN plasmids carrying qnrS1, qnrB2 and qnrB19 genes were identified in Salmonella enterica Bredeney, Typhimurium PT507, Kentucky and Saintpaul. qnrS1 genes were also located on three further plasmid types, belonging to the ColE (in Salmonella Corvallis and Anatum), IncR (in Salmonella Montevideo) and IncHI2 (in Salmonella Stanley) groups. Multiple events of mobilization, transposition and replicon fusion generate the complexity observed in qnr-positive isolates that are emerging worldwide. Despite the fact that the occurrence of qnr genes in bacteria from animals is scarcely reported, these genes are associated with genetic elements and located on plasmids that are recurrent in animal isolates.Keywords
This publication has 31 references indexed in Scilit:
- Dissemination of ESBL and Qnr determinants in Enterobacter cloacae in AlgeriaJournal of Antimicrobial Chemotherapy, 2008
- First report of qnr genes in Salmonella in The NetherlandsJournal of Antimicrobial Chemotherapy, 2007
- Complete nucleotide sequence of a small qnrS1-carrying plasmid from Salmonella enterica subsp. enterica Typhimurium DT193Journal of Antimicrobial Chemotherapy, 2007
- Plasmid-mediated quinolone resistance determinant qnrS1 found in Salmonella enterica strains isolated in the UKJournal of Antimicrobial Chemotherapy, 2007
- Prevalence of qnr genes in Salmonella in FranceJournal of Antimicrobial Chemotherapy, 2007
- Complete Nucleotide Sequence of pK245, a 98-Kilobase Plasmid Conferring Quinolone Resistance and Extended-Spectrum-β-Lactamase Activity in a Clinical Klebsiella pneumoniae IsolateAntimicrobial Agents and Chemotherapy, 2006
- The worldwide emergence of plasmid-mediated quinolone resistanceThe Lancet Infectious Diseases, 2006
- Plasmid‐Mediated Quinolone Resistance in Non‐Typhi Serotypes ofSalmonella entericaClinical Infectious Diseases, 2006
- Identification of the plasmid-borne quinolone resistance gene qnrS in Salmonella enterica serovar InfantisJournal of Antimicrobial Chemotherapy, 2006
- Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferaseNature Medicine, 2005