High degree of genetic variation inProchlorococcus(Prochlorophyta) revealed by RFLP analysis
Open Access
- 1 February 1996
- journal article
- research article
- Published by Informa UK Limited in European Journal of Phycology
- Vol. 31 (1), 1-9
- https://doi.org/10.1080/09670269600651131
Abstract
A genetic characterization of nine strains of Prochlorococcus originating from various depths of the Mediterranean Sea, the Sargasso Sea, the North and the tropical Atlantic Ocean and the Pacific Ocean was performed by restriction fragment length polymorphism (RFLP) mapping using probes for rbcL, rbcS, psbA and woxA. This study revealed extensive genetic variation among strains, which were grouped into two distinct clusters. Unexpectedly, with one exception (TATL1 strain), the strains clustered by isolation depth (i.e. near surface versus deep isolates) rather than by geographic origin. These relationships were confirmed by secondary RFLP analysis of psbA amplification products using Prochlorococcus-specific polymerase chain reaction (PCR) primers.Keywords
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