RNA circularization strategies in vivo and in vitro
Top Cited Papers
Open Access
- 6 February 2015
- journal article
- review article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 43 (4), 2454-2465
- https://doi.org/10.1093/nar/gkv045
Abstract
In the plenitude of naturally occurring RNAs, circular RNAs (circRNAs) and their biological role were underestimated for years. However, circRNAs are ubiquitous in all domains of life, including eukaryotes, archaea, bacteria and viruses, where they can fulfill diverse biological functions. Some of those functions, as for example playing a role in the life cycle of viral and viroid genomes or in the maturation of tRNA genes, have been elucidated; other putative functions still remain elusive. Due to the resistance to exonucleases, circRNAs are promising tools for in vivo application as aptamers, trans-cleaving ribozymes or siRNAs. How are circRNAs generated in vivo and what approaches do exist to produce ring-shaped RNAs in vitro? In this review we illustrate the occurrence and mechanisms of RNA circularization in vivo, survey methods for the generation of circRNA in vitro and provide appropriate protocols.Keywords
This publication has 118 references indexed in Scilit:
- RNA ligase RtcB splices 3′-phosphate and 5′-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3′)pp(5′)G intermediatesProceedings of the National Academy of Sciences of the United States of America, 2012
- Transcriptome-wide discovery of circular RNAs in ArchaeaNucleic Acids Research, 2011
- miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNAThe EMBO Journal, 2011
- Hepatitis delta virusThe Lancet, 2011
- RtcB Is the RNA Ligase Component of an Escherichia coli RNA Repair OperonPublished by Elsevier BV ,2011
- A natural ribozyme with 3′,5′ RNA ligase activityNature Chemical Biology, 2009
- Hairpin ribozyme-antisense RNA constructs can act as molecular lassosNucleic Acids Research, 2008
- RNA LigasesCurrent Protocols in Molecular Biology, 2008
- Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4Biochemical Journal, 2006
- Multiple physical forms of excised group II intron RNAs in wheat mitochondriaNucleic Acids Research, 2006