PAIR3, an axis-associated protein, is essential for the recruitment of recombination elements onto meiotic chromosomes in rice
Open Access
- 1 January 2011
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 22 (1), 12-19
- https://doi.org/10.1091/mbc.e10-08-0667
Abstract
During meiosis, the paired homologous chromosomes are tightly held together by the synaptonemal complex (SC). This complex consists of two parallel axial/lateral elements (AEs/LEs) and one central element. Here, we observed that PAIR3 localized to the chromosome core during prophase I and associated with both unsynapsed AEs and synapsed LEs. Analyses of the severe pair3 mutant demonstrated that PAIR3 was essential for bouquet formation, homologous pairing and normal recombination, and SC assembly. In addition, we showed that although PAIR3 was not required for the initial recruitment of PAIR2, it was required for the proper association of PAIR2 with chromosomes. Dual immunostaining revealed that PAIR3 highly colocalized with REC8. Moreover, studies using a rec8 mutant indicated that PAIR3 localized to chromosomes in a REC8-dependent manner.Keywords
This publication has 43 references indexed in Scilit:
- The Central Element Protein ZEP1 of the Synaptonemal Complex Regulates the Number of Crossovers during Meiosis in RicePlant Cell, 2010
- Interlock Formation and Coiling of Meiotic Chromosome Axes During SynapsisGenetics, 2009
- Pds5 is required for homologue pairing and inhibits synapsis of sister chromatids during yeast meiosisThe Journal of cell biology, 2009
- The axial element protein HTP-3 promotes cohesin loading and meiotic axis assembly in C. elegans to implement the meiotic program of chromosome segregationGenes & Development, 2009
- Maize AMEIOTIC1 is essential for multiple early meiotic processes and likely required for the initiation of meiosisProceedings of the National Academy of Sciences of the United States of America, 2009
- HTP-3 Links DSB Formation with Homolog Pairing and Crossing Over during C. elegans MeiosisDevelopmental Cell, 2008
- ASY1 mediates AtDMC1-dependent interhomolog recombination during meiosis in ArabidopsisGenes & Development, 2007
- Mouse SYCP2 is required for synaptonemal complex assembly and chromosomal synapsis during male meiosisThe Journal of cell biology, 2006
- A bouquet of chromosomesJournal of Cell Science, 2004
- An insertional mutation in the rice PAIR2 gene, the ortholog of Arabidopsis ASY1, results in a defect in homologous chromosome pairing during meiosisMolecular Genetics and Genomics, 2004