Proteomic analysis of dentin–enamel junction and adjacent protein‐containing enamel matrix layer of healthy human molar teeth
- 22 November 2018
- journal article
- research article
- Published by Wiley in European Journal of Oral Sciences
- Vol. 127 (2), 112-121
- https://doi.org/10.1111/eos.12594
Abstract
The dentin–enamel junction (DEJ) is the border where two different mineralized structures – enamel and dentin – meet. The protein‐rich DEJ, together with the inner enamel region of mature teeth, is known to exhibit higher fracture toughness and crack growth resistance than bulk phase enamel. However, an explanation for this behavior has been hampered by the lack of compositional information for the DEJ and the adjacent enamel organic matrix (EOM). We studied proteomes of the DEJ and EOM of healthy human molars and compared them with dentin and enamel proteomes from the same teeth. These tissues were cut out of tooth sections by laser capture microdissection, proteins were extracted and cleaved by trypsin, then processed by liquid chromatography coupled to tandem mass spectrometry to analyze the proteome profiles of these tissues. This study identified 46 proteins in DEJ and EOM. The proteins identified have a variety of functions, including calcium ion‐binding, formation of extracellular matrix, formation of cytoskeleton, cytoskeletal protein binding, cell adhesion, and transport. Collagens were identified as the most dominant proteins. Tissue‐specific proteins, such as ameloblastin and amelogenin, were also detected. Our findings reveal new insight into proteomics of DEJ and EOM, highly mineralized tissues that are obviously difficult to analyze.Keywords
Funding Information
- Grantová Agentura České Republiky (17‐10832S)
This publication has 47 references indexed in Scilit:
- Laser capture microdissection enables cellular and molecular studies of tooth root developmentInternational Journal of Oral Science, 2012
- Comprehensive analysis of gene expression in the junctional epithelium by laser microdissection and microarray analysisJournal of Periodontal Research, 2010
- Expression Analysis of Human Salivary Glands by Laser Microdissection: Differences Between Submandibular and Labial GlandsCellular Physiology and Biochemistry, 2010
- Individual Variations in Protective Effects of Experimentally Formed Salivary PelliclesCaries Research, 2009
- Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTipsNature Protocols, 2007
- Two‐dimensional electrophoresis study of in vitro pellicle formation and dental caries susceptibilityEuropean Journal of Oral Sciences, 2006
- The Dentino-Enamel Junction RevisitedConnective Tissue Research, 2002
- The Dentino-Enamel Junction RevisitedConnective Tissue Research, 2002
- The Dentino‐enamel Junction is a Broad Transitional Zone Uniting Dissimilar Bioceramic CompositesJournal of the American Ceramic Society, 2000
- COLLAGENS: Molecular Biology, Diseases, and Potentials for TherapyAnnual Review of Biochemistry, 1995