Environmentally friendly LC for the simultaneous determination of ascorbic acid and its derivatives in skin‐whitening cosmetics
- 1 February 2008
- journal article
- research article
- Published by Wiley in Journal of Separation Science
- Vol. 31 (2), 229-236
- https://doi.org/10.1002/jssc.200700414
Abstract
Ascorbic acid (AA), also known as vitamin C, is a very popular skin‐whitening agent used in cosmetics. However, the use of AA (and also its sodium or magnesium salts) in cosmetic products is limited owing to its labile oxidative properties. In order to avoid its early degradation, different derivatives have been designed, such as ascorbyl phosphate (APH; as magnesium or sodium salts) and ascorbyl palmitate (AP), and more recently the ascorbyl glucoside (AG). Bearing in mind that all these chemicals in skin‐whitening cosmetic products must be determined in order to control the efficacy of such products, this paper focuses on developing a wide‐ranging LC analytical method able to determine the above‐mentioned compounds simultaneously in cosmetic products. The chromatographic variables were studied and selected in order to achieve the total separation and subsequent determination of all the analytes involved. Thus, an octadecylsilica (C18) stationary phase and a mobile phase gradient of ethanol: 50 mM phosphate buffer at different pHs (containing 0.1 M NaCl) were used. Detection was carried out with a UV/visible spectrometry detector set at different wavelengths. The LOD ranged from 2 to 6 μg/mL depending on the analyte. The proposed method was validated by analysing a laboratory‐made and six commercial skin‐whitening cosmetic samples. The method allows any mixture of the four skin‐whitening agents studied to be both separated at good resolution and determined without interferences from samples, and moreover it does not require the use of either highly toxic organic solvents or hazardous chemicals.Keywords
This publication has 26 references indexed in Scilit:
- Validation of HPLC stability-indicating method for Vitamin C in semisolid pharmaceutical/cosmetic preparations with glutathione and sodium metabisulfite, as antioxidantsTalanta, 2007
- Combining high-performance liquid chromatography with on-line microdialysis sampling for the simultaneous determination of ascorbyl glucoside, kojic acid, and niacinamide in bleaching cosmeticsAnalytica Chimica Acta, 2007
- Melasma and its impact on health‐related quality of life in Hispanic womenJournal of Dermatological Treatment, 2007
- Development and validation of a reversed-phase ion-pair liquid chromatography method for the determination of magnesium ascorbyl phosphate and melatonin in cosmetic creamsAnalytica Chimica Acta, 2006
- Simultaneous determination of l-ascorbic acid, ascorbic acid-2-phosphate magnesium salt, and ascorbic acid-6-palmitate in commercial cosmetics by micellar electrokinetic capillary electrophoresisAnalytica Chimica Acta, 2006
- An environmentally friendly (“green”) reversed-phase liquid chromatography method for UV filters determination in cosmeticsAnalytica Chimica Acta, 2005
- A double‐blind randomized trial of 5% ascorbic acid vs. 4% hydroquinone in melasmaInternational Journal of Dermatology, 2004
- Topical Activity of Ascorbic Acid: From in vitro Optimization to in vivo EfficacySkin Pharmacology and Physiology, 2004
- Skin‐lightening products revisitedInternational Journal of Cosmetic Science, 2003
- Stability of vitamin C derivatives in solution and topical formulationsJournal of Pharmaceutical and Biomedical Analysis, 1997