Development and evaluation of a real-time polymerase chain reaction method for the detection ofMycoplasma felis
Open Access
- 15 June 2011
- journal article
- Published by SAGE Publications in Journal of Veterinary Diagnostic Investigation
- Vol. 23 (5), 890-893
- https://doi.org/10.1177/1040638711407479
Abstract
Infection by Mycoplasma felis is associated with ocular and respiratory disease in cats and respiratory disease in horses. A correct diagnosis is beneficial since the use of specific antimycoplasmal treatment can lead to resolution. The objective of the present study was to develop a real-time polymerase chain reaction (PCR) method based on dual-labeled fluorogenic probe technology, targeting the gene encoding elongation factor Tu (tuf ), for the fast and specific detection of M. felis. Specificity was achieved by basing the assay design on partial sequencing of the tuf gene in strains and clinical isolates of M. felis as well as other mycoplasma species. The detection limit of the developed assay was in the order of 10 copies of target DNA, and no cross-reaction was observed with a panel of several mycoplasma species. Compared to a previously published conventional PCR protocol, the novel assay had equal or slightly improved performance in terms of sensitivity and specificity when analyzing 100 conjunctival swab samples from cats with clinical signs of infection.Keywords
This publication has 21 references indexed in Scilit:
- Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an ExampleApplied and Environmental Microbiology, 2008
- Efficacy of Pradofloxacin in Cats with Feline Upper Respiratory Tract Disease due to Chlamydophila felis or Mycoplasma InfectionsJournal of Veterinary Internal Medicine, 2008
- Mycoplasma felisarthritis in two catsJournal of Small Animal Practice, 2006
- Assessment of infectious organisms associated with chronic rhinosinusitis in catsJournal of the American Veterinary Medical Association, 2005
- Clinical Use of 16S rRNA Gene Sequencing To IdentifyMycoplasma felisandM. gateaeAssociated with Feline Ulcerative KeratitisJournal of Clinical Microbiology, 2005
- Epidemiologic evaluation of multiple respiratory pathogens in cats in animal sheltersJournal of Feline Medicine and Surgery, 2005
- Development of a polymerase chain reaction for the detection of Mycoplasma felis in domestic catsVeterinary Microbiology, 2004
- Incidence and significane of isolation of Mycoplasma felis from conjunctival swabs of catsVeterinary Microbiology, 1991
- Pulse-field electrophoresis indicates full-length mycoplasma chromosomes range widely in sizeNucleic Acids Research, 1990
- EQUINE MYCOPLASMASPublished by Elsevier BV ,1979