On the mechanism of action of carcinogenic aromatic amines: I. Binding of 2-acetylaminofluorene and n-hydroxy-2-acetylaminofluorene to rat-liver nucleic acids in vivo

Abstract

The binding of AAF and AF to rat-liver nucleic acids in vivo has been studied 24 h after intraperitoneal injection of [9-¹⁴C], [9-¹⁴C]AAF, N-hydroxy-[9-¹⁴C]AAF and N-hydroxy-N-[1'-¹⁴C] acetyl-AF in equal doses. The hepatic nucleic acids were hydrolyzed at pH 9 with a mixture of venom phosphodiesterase and alkaline phosphatase from E. coli. The guanosine-bound carcinogens were isolated from the nucleic acid hydrolysates with a new Chromatographic procedure using columns of lipophilic Sephadex LH-20.