DJ‐1, a causative gene product of a familial form of Parkinson's disease, is secreted through microdomains
- 27 June 2008
- journal article
- Published by Wiley in FEBS Letters
- Vol. 582 (17), 2643-2649
- https://doi.org/10.1016/j.febslet.2008.06.043
Abstract
DJ-1 is secreted into the serum and plasma of patients with various diseases. In this study, DJ-1 was found to be secreted into culture media of various cells and the amount of wild-type DJ-1 secreted was two-fold greater than that of mutant DJ-1 of cysteine at 106 (C106). Furthermore, the oxidative status of more than 90% of the DJ-1 secreted from HeLa cells was SOH and SO2H forms of C106. A portion of DJ-1 in cells was localized in microdomains of the membrane. These findings suggest that DJ-1 is secreted through microdomains and that oxidation of DJ-1 at C106 facilitates the secretion.Keywords
This publication has 34 references indexed in Scilit:
- Calcium microdomains and cell cycle controlCell Calcium, 2006
- DJ-1, a cancer- and Parkinson's disease-associated protein, stabilizes the antioxidant transcriptional master regulator Nrf2Proceedings of the National Academy of Sciences of the United States of America, 2006
- Specificity in reactive oxidant signaling: think globally, act locallyThe Journal of cell biology, 2006
- Increased level of DJ-1 in the cerebrospinal fluids of sporadic Parkinson’s diseaseBiochemical and Biophysical Research Communications, 2006
- The Oxidation State of DJ-1 Regulates its Chaperone Activity Toward α-SynucleinJournal of Molecular Biology, 2005
- Sensitivity to Oxidative Stress in DJ-1-Deficient Dopamine Neurons: An ES- Derived Cell Model of Primary ParkinsonismPLoS Biology, 2004
- Reduced anti-oxidative stress activities of DJ-1 mutants found in Parkinson’s disease patientsBiochemical and Biophysical Research Communications, 2004
- Cysteine-106 of DJ-1 is the most sensitive cysteine residue to hydrogen peroxide-mediated oxidation in vivo in human umbilical vein endothelial cellsBiochemical and Biophysical Research Communications, 2004
- DJ‐1 has a role in antioxidative stress to prevent cell deathEMBO Reports, 2004
- DJ-1, a Novel Oncogene Which Transforms Mouse NIH3T3 Cells in Cooperation withrasBiochemical and Biophysical Research Communications, 1997