Aspects of Salt Tolerance in a NaCl-Selected Stable Cell Line of Citrus sinensis
Open Access
- 1 July 1983
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 72 (3), 685-690
- https://doi.org/10.1104/pp.72.3.685
Abstract
A NaCl-tolerant cell line which was selected from ovular callus of `Shamouti' orange (Citrus sinensis L. Osbeck) proved to be a true cell line variant. This conclusion is based on the following observations. (a) Cells which have been removed from the selection pressure for at least four passages retain the same NaCl tolerance as do cells which are kept constantly on 0.2 molar NaCl. (b) Na+ and Cl− uptake are considerably lower in salt-tolerant cells (R-10) than in salt-sensitive cells (L-5) at a given external NaCl concentration. (c) Growth of salt-tolerant cells is markedly suppressed upon replacement of NaCl by KCl, whereas the growth of salt-sensitive cells is only slightly affected. Accumulation of K+ and Cl− accompanies the inhibition of growth. Experiments carried out with sodium and potassium sulfate suggest that the toxic effect is due to the accumulated Cl−. (d) Removal of Ca2+ from the growth medium severely inhibits the growth of salt-tolerant cells in the presence of NaCl, while it has a minor effect on growth of salt-sensitive cells in the presence of NaCl. (e) Electron micrographs show that the salt-tolerant cells have very big vacuoles when exposed to salt, while the size of the vacuoles of the salt-sensitive cells does not change.Keywords
This publication has 4 references indexed in Scilit:
- Osmotic Adjustment of Cultured Tobacco Cells (Nicotiana tabacum var. Samsum) Grown on Sodium ChloridePlant Physiology, 1981
- Direct selection in vitro for herbicide-resistant mutants of Nicotiana tabacumProceedings of the National Academy of Sciences of the United States of America, 1978
- SOMATIC CELL GENETICS OF HIGHER PLANTSAnnual Review of Genetics, 1974
- A modified ninhydrin colorimetric analysis for amino acidsArchives of Biochemistry and Biophysics, 1957