Study of cell kinetics in anaplastic thyroid carcinoma transplanted to nude mice
- 1 May 1989
- journal article
- research article
- Published by Wiley in Journal of Surgical Oncology
- Vol. 41 (1), 1-4
- https://doi.org/10.1002/jso.2930410104
Abstract
The cell kinetics of the transplantable human anaplastic thyroid carcinoma were studied in athymic nude mice using bromodeoxyuridine (BrdU) pulse labeling. In order to estimate the cell cycle time in this study, the percent labeled mitosis curve was prepared with immunoperoxidase staining using monoclonal antibody to BrdU instead of 3[H]thymidine autoradiography. This technique was considered to be very useful because it can be done for a short term and can still be changed to the autoradiographic method. Comparing the results in this study with already reported data on various xenografts of human carcinomas in nude mice, the tumor doubling time (117.5 hr) and the cell cycle time (23.5 hr) were rather short, but the growth fraction (54%) and the cell loss factor (68%) were nearly equivalent to the median value. These findings suggest that the very rapid growth rate of this tumor was mainly due to the shortness of the cell cycle time.This publication has 15 references indexed in Scilit:
- A randomized trial of doxorubicin versus doxorubicin plus cisplatin in patients with advanced thyroid carcinomaCancer, 1985
- Bromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody.JCI Insight, 1983
- Treatment of anaplastic giant and spindle cell carcinoma of the thyroid gland with combination adriamycin and radiation therapy a new approachCancer, 1983
- Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA ReplicationScience, 1982
- Transplantation of Human Tumors in Nude Mice23JNCI Journal of the National Cancer Institute, 1976
- Chemotherapeutic implications of growth fraction and cell cycle time in glioblastomasJournal of Neurosurgery, 1975
- Spindle and giant cell carcinoma of the thyroid: A different therapeutic approachCancer, 1974
- HETEROTRANSPLANTATION OF A HUMAN MALIGNANT TUMOUR TO “NUDE” MICEActa Pathologica Microbiologica Scandinavica, 1969
- The relationship between the duration of the S phase and the fraction of cells which incorporate 3H-thymidine during exponential growthExperimental Cell Research, 1965
- Cell population kinetics in the intestinal epithelium of the mouseExperimental Cell Research, 1959